Abstract | OBJECTIVE: METHODS: A total of 101 recent, histologically or clinically confirmed malignant effusions with immunostained cell block preparations were reviewed. The cases consisted of 34 mesotheliomas and 67 adenocarcinomas. This included 17 ascitic fluid and 84 pleural fluid samples. The adenocarcinomas included metastatic carcinomas from the breast (12), lung (19), stomach (3), colon (1), pancreas (2), ovary (6) endometrium (1) and 23 histologically confirmed metastases from unknown primary sites. The cases were assessed as negative or positive (>5% of cells stained). The staining pattern was recorded as cytoplasmic, cell membrane, nuclear or cytoplasmic and nuclear staining. RESULTS: CONCLUSIONS: The results confirm that calretinin and CK 5/6 are useful markers for mesothelioma in effusion specimens. CK5/6 staining may be less useful for peritoneal fluid specimens where metastatic adenocarcinomas may be more likely to express the antigen. Further study of ascitic/peritoneal specimens is warranted. However, positive staining, particularly for both antigens, is highly indicative of a mesothelial origin for cells. The two markers make a useful addition to EMA and the panel of adenocarcinoma markers routinely applied to effusion specimens.
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Authors | P W Shield, K Koivurinne |
Journal | Cytopathology : official journal of the British Society for Clinical Cytology
(Cytopathology)
Vol. 19
Issue 4
Pg. 218-23
(Aug 2008)
ISSN: 1365-2303 [Electronic] England |
PMID | 17916095
(Publication Type: Journal Article)
|
Chemical References |
- Biomarkers, Tumor
- CALB2 protein, human
- Calbindin 2
- Keratin-5
- Keratin-6
- S100 Calcium Binding Protein G
|
Topics |
- Biomarkers, Tumor
(analysis)
- Calbindin 2
- Carcinoma
(diagnosis)
- Diagnosis, Differential
- Humans
- Keratin-5
(analysis)
- Keratin-6
(analysis)
- Mesothelioma
(diagnosis)
- Neoplasms
(diagnosis)
- Pleural Effusion
(pathology)
- Reproducibility of Results
- S100 Calcium Binding Protein G
(analysis)
- Sensitivity and Specificity
- Staining and Labeling
|