Abstract | BACKGROUND: MATERIALS AND METHODS: We used the breast cancer cell lines MDA-MB-231 and BT-483. Cell inhibition rate was measured by WST-1 reagent, cell apoptosis by Apoptotic DNA Ladder Kit, mRNA expression of MMP-1, MMP-3 and MMP-9 by PCR and TERT by immunohistochemistry stain. RESULTS: Comparing to the control group, the test group showed lower cell growth rate, decreased mRNA expression of MMP-1, MMP-3 and MMP-9 production and less intense staining of MMPs with diaminobenzidine tetrahydrochloride. CONCLUSION:
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Authors | Wings T Y Loo, J P Chen, Louis W C Chow, Jeffrey W K Chou |
Journal | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
(Biomed Pharmacother)
Vol. 61
Issue 9
Pg. 601-5
(Oct 2007)
ISSN: 0753-3322 [Print] France |
PMID | 17913446
(Publication Type: Journal Article)
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Chemical References |
- 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium
- Drugs, Chinese Herbal
- Matrix Metalloproteinase Inhibitors
- Protease Inhibitors
- RNA, Messenger
- RNA, Neoplasm
- Tetrazolium Salts
- shugansanjie tang
- Telomerase
- Matrix Metalloproteinases
- Matrix Metalloproteinase 3
- Matrix Metalloproteinase 9
- Matrix Metalloproteinase 1
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Topics |
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy, enzymology, pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Drugs, Chinese Herbal
(pharmacology)
- Female
- Humans
- Immunohistochemistry
- In Situ Nick-End Labeling
- Matrix Metalloproteinase 1
(biosynthesis)
- Matrix Metalloproteinase 3
(biosynthesis)
- Matrix Metalloproteinase 9
(biosynthesis)
- Matrix Metalloproteinase Inhibitors
- Matrix Metalloproteinases
(biosynthesis)
- Protease Inhibitors
- RNA, Messenger
(biosynthesis, genetics)
- RNA, Neoplasm
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Telomerase
(biosynthesis)
- Tetrazolium Salts
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