Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs.

Abstract

Cellular and humoral immune responses of dogs to a candidate vaccine, composed of Leishmania braziliensis promastigote protein plus saponin as adjuvant, have been investigated as a pre-requisite to understanding the mechanisms of immunogenicity against canine visceral leishmaniasis (CVL). The candidate vaccine elicited strong antigenicity related to the increases of anti-Leishmania IgG isotypes, together with higher levels of lymphocytes, particularly of circulating CD8(+) T-lymphocytes and Leishmania chagasi antigen-specific CD8(+) T-lymphocytes. As indicated by the intense cell proliferation and increased nitric oxide production during in vitro stimulation by L. chagasi soluble antigens, the candidate vaccine elicited an immune activation status potentially compatible with effective control of the etiological agent of CVL.

• Authors: Rodolfo Cordeiro Giunchetti, Rodrigo Corrêa-Oliveira, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho, Bruno Mendes Roatt, Rodrigo Dian de Oliveira Aguiar-Soares, Juliana Vitoriano de Souza, Nádia das Dores Moreira, Luiz Cosme Cotta Malaquias, Luciana Lisboa Mota e Castro, Marta de Lana, Alexandre Barbosa Reis
• Journal: Vaccine (Vaccine) Vol. 25 Issue 44 Pg. 7674-86 (Nov 01 2007) ISSN: 0264-410X [Print] Netherlands
• PMID: 17913311 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Protozoan
• Protozoan Vaccines
• Saponins
• Vaccines, Inactivated
• Nitric Oxide

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antibodies, Protozoan (blood)
• B-Lymphocytes (immunology)
• Dog Diseases (prevention & control)
• Dogs
• Female
• Leishmania braziliensis (immunology)
• Leishmaniasis, Visceral (prevention & control, veterinary)
• Lymphocyte Activation
• Male
• Nitric Oxide (biosynthesis)
• Protozoan Vaccines (immunology)
• Saponins (administration & dosage)
• T-Lymphocyte Subsets (immunology)
• Vaccines, Inactivated