Abstract |
In this study, we have investigated the potential of trans-activating transcriptional activator peptide (TATp)-modified liposomes to enhance the delivery of the model gene, plasmid encoding for the green fluorescent protein (pEGFP-N1), to human brain tumor U-87 MG cells in vitro and in an intracranial model in nude mice. The TATp-lipoplexes were characterized at lipid/ DNA (+/-) charge ratios of 0.2, 5, 10, and 20 for size analysis and DNA complexation. The size distribution of DNA-loaded TATp- liposomes was narrow and the DNA complexation was firm at lipid/ DNA (+/-) charge ratios of 5 and higher. TATp-lipoplexes had demonstrated an enhanced delivery of pEGFP-N1 to U-87 MG tumor cells in vitro at lipid/ DNA (+/-) charge ratios of 5 and 10. In vivo transfection of intracranial brain tumors by intratumoral injections of TATp-lipoplexes showed an enhanced delivery of pEGFP-N1 selectively to tumor cells and subsequent effective transfection compared to plain plasmid-loaded lipoplexes. No transfection (green fluorescence of the GFP) was noted in the normal brain adjacent to tumor.
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Authors | Bhawna Gupta, Tatiana S Levchenko, Vladimir P Torchilin |
Journal | Oncology research
(Oncol Res)
Vol. 16
Issue 8
Pg. 351-9
( 2007)
ISSN: 0965-0407 [Print] United States |
PMID | 17913043
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Gene Products, tat
- Liposomes
- Green Fluorescent Proteins
- DNA
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Topics |
- Animals
- Astrocytoma
(pathology, therapy)
- Brain Neoplasms
(pathology, therapy)
- Cerebral Cortex
- DNA
(administration & dosage, genetics)
- Drug Administration Routes
- Gene Products, tat
(administration & dosage)
- Gene Transfer Techniques
- Genes, tat
(genetics)
- Genetic Therapy
(methods)
- Green Fluorescent Proteins
(genetics)
- Humans
- Injections, Intralesional
- Liposomes
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Transfection
- Transplantation, Heterologous
- Tumor Cells, Cultured
- Up-Regulation
- Xenograft Model Antitumor Assays
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