D-003 does not possess oestrogenic potential in-vivo: findings of the uterotrophic assay.

Abstract

D-003 is a mixture of long-chain fatty acids purified from sugarcane wax that inhibits both cholesterol synthesis prior to mevalonate formation, and lipid peroxidation. D-003 has been shown to prevent bone loss and bone resorption in ovariectomized rats, and significantly improves bone resorption markers in postmenopausal women with reduced bone mineral density. As hormone-replacement therapy, D-003 displays cholesterol-lowering and anti-resorptive effects. We have studied its potential oestrogenic activity in-vivo using the uterotrophic assay. Rats were randomly distributed into five groups: a sham-operated group and four groups of ovariectomized rats, one treated with vehicle, one with D-003 (50 mg kg(-1)), one with oestradiol benzoate (30 microg kg(-1)) and one with D-003 (50 mg kg(-1)) plus oestradiol benzoate (30 microg kg(-1)). Treatments were administered for 14 days. Ovariectomy decreased the values of relative uterus weight, epithelium cell height and endometrial thickness compared with sham-operated rats, and these effects were all significantly reduced with oestradiol benzoate, but not with D-003. Concurrent administration of D-003 and oestradiol benzoate had statistically similar effects on all variables as oestradiol benzoate alone. In conclusions, D-003 orally given at 50 mg kg(-1), a dose that prevents bone loss and bone resorption in ovariectomized rats, did not display oestrogenic/anti-oestrogenic activity in-vivo, as assessed in the uterotrophic assay.

• Authors: Miriam Noa, Sarahí Mendoza, Rosa Mas, Rafael Gámez, Maikel Valle, Balia Pardo, Ariadne Gutiérrez, Nilda Mendoza
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 59 Issue 10 Pg. 1433-7 (Oct 2007) ISSN: 0022-3573 [Print] England
• PMID: 17910820 (Publication Type: Comparative Study, Journal Article)

Chemical References

• D-003 sugarcane wax acid mixture
• Fatty Acids
• estradiol 3-benzoate
• Estradiol

Topics

• Administration, Oral
• Animals
• Body Weight (drug effects)
• Endometrium (drug effects, metabolism)
• Epithelial Cells (drug effects, metabolism)
• Estradiol (analogs & derivatives, pharmacology)
• Fatty Acids (pharmacology)
• Female
• Organ Size (drug effects)
• Ovariectomy
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Uterus (drug effects, metabolism)