Abstract | AIM: METHODS: RESULTS: Multicellular spheroids exhibited structural characteristics somewhat different to those in monolayers. The cells in three-dimensional cell culture turned out to be less sensitive to delisheng, 5-fluorouracil and adriamycin than the cells cultured in monolayer. This showed that delisheng had a satisfactory cells inhibition ratio compared to 5-fluorouracil and adriamycin. Immunohistochemical staining showed that VEGF and endostatin expression was positive during growth as multicellular spheroids, and endostatin expression in spheroids with treatment of delisheng was higher than that with 5-fluorouracil, adriamycin and PBS (139.35 +/- 7.83, 159.23 +/- 10.34, 162.83 +/- 3.47 and 148.48 +/- 11.06, P < 0.05). CONCLUSION: Chinese medicine compound delisheng has satisfactory anti- tumor activity in HepG2 cells in three-dimensional culture, and the effects are associated with up-regulation of endostatin.
|
Authors | Jie Cui, Ke-Jun Nan, Tao Tian, Ya-Huan Guo, Na Zhao, Lin Wang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 13
Issue 41
Pg. 5432-9
(Nov 07 2007)
ISSN: 1007-9327 [Print] United States |
PMID | 17907285
(Publication Type: Comparative Study, Journal Article)
|
Chemical References |
- Antineoplastic Agents
- Antineoplastic Agents, Phytogenic
- Drugs, Chinese Herbal
- Endostatins
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
- delisheng
- Doxorubicin
- Fluorouracil
|
Topics |
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Carcinoma, Hepatocellular
(drug therapy, metabolism, pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Shape
- Dose-Response Relationship, Drug
- Doxorubicin
(pharmacology, therapeutic use)
- Drug Resistance, Neoplasm
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Endostatins
(biosynthesis)
- Fluorouracil
(pharmacology, therapeutic use)
- Humans
- Immunohistochemistry
- Liver Neoplasms
(drug therapy, metabolism, pathology)
- Microscopy, Electron, Scanning
- Microscopy, Electron, Transmission
- Microscopy, Phase-Contrast
- Spheroids, Cellular
- Time Factors
- Up-Regulation
- Vascular Endothelial Growth Factor A
(metabolism)
|