Abstract | RATIONALE: OBJECTIVES: To establish if sPLA(2)s are overexpressed in the airways of patients with asthma, and to determine if these enzymes may play a role in the generation of eicosanoids in exercise-induced bronchoconstriction. METHODS: Induced sputum samples were obtained from subjects with asthma with exercise-induced bronchoconstriction and nonasthmatic control subjects at baseline, and on a separate day 30 minutes after exercise challenge. The expression of the PLA(2)s in induced sputum cells and supernatant was determined by quantitative polymerase chain reaction, immunocytochemistry, and Western blot. MEASUREMENTS AND MAIN RESULTS: The sPLA(2)s expressed at the highest levels in airway cells of subjects with asthma were groups X and XIIA. Group X sPLA(2) (sPLA(2)-X) was differentially overexpressed in asthma and localized to airway epithelial cells and bronchial macrophages. The gene expression, immunostaining in airway epithelial cells and bronchial macrophages, and the level of the extracellular sPLA(2)-X protein in the airways increased in response to exercise challenge in the asthma group, whereas the levels were lower and unchanged after challenge in nonasthmatic control subjects. CONCLUSIONS:
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Authors | Teal S Hallstrand, Emil Y Chi, Alan G Singer, Michael H Gelb, William R Henderson Jr |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 176
Issue 11
Pg. 1072-8
(Dec 01 2007)
ISSN: 1535-4970 [Electronic] United States |
PMID | 17901411
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Eicosanoids
- Isoenzymes
- Phospholipases A2, Secretory
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Topics |
- Adolescent
- Adult
- Asthma
(enzymology, metabolism, physiopathology)
- Bronchi
(enzymology)
- Bronchial Hyperreactivity
(enzymology, etiology, metabolism, physiopathology)
- Bronchoconstriction
- Cohort Studies
- Eicosanoids
(analysis)
- Epithelial Cells
(enzymology)
- Exercise
- Female
- Humans
- Isoenzymes
(metabolism)
- Male
- Middle Aged
- Phospholipases A2, Secretory
(classification, metabolism)
- Respiratory System
(enzymology)
- Sputum
(chemistry, cytology)
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