In our previous study, we found that
cycloheximide (CHX) induces hepatocellular
necrosis as well as hepatocellular apoptosis. This article evaluates the role of Kupffer cells on
cycloheximide-induced hepatic injury using
gadolinium chloride (GdCl(3)) for the inhibition of Kupffer cells. One group of rats was treated with CHX (CHX group), and another was treated with GdCl(3) before being treated with the same dose of CHX (GdCl(3)/CHX group). The necrotic change in the GdCl(3)/CHX group was exacerbated under the induction of hepatocellular apoptosis by the CHX treatment. A substantial diminution of the number of ED1- or ED2-positive cells was demonstrated in the GdCl(3)/CHX group compared to the CHX group. In addition, the degree of decrease in ED2-positive cells was more apparent than that in ED1-positive cells. Increases in the
mRNA levels of
IL-10 and Stat3 were observed in the CHX group, but not in the GdCl(3)/CHX group. On the other hand, the hepatic
mRNA levels of
chemokines and adhesion molecules such as Ccl20, LOX-1, and
E-selectin were significantly increased only in the GdCl(3)/CHX group. Thus, Kupffer cell inactivation by the GdCl(3) treatment leads to a loss of the capacity to produce
IL-10, supposedly resulting in the enhancement of pro-inflammatory
cytokine activities such as
tumor necrosis factor (TNF) signaling. These events are suggested to be
a factor of the inflammatory exacerbation in the livers of the GdCl(3)/CHX group. In conclusion, Kupffer cells may play a role in protecting hepatic necroinflammatory changes by releasing anti-inflammatory
cytokines following the hepatocellular apoptosis resulting from CHX treatment.