Abstract |
KEG1/YFR042w of Saccharomyces cerevisiae is an essential gene that encodes a 200-amino acid polypeptide with four predicted transmembrane domains. The green fluorescent protein- or Myc(6)-tagged Keg1 protein showed the typical characteristics of an integral membrane protein and was found in the endoplasmic reticulum by fluorescence imaging. Immunoprecipitation from the Triton X-100-solubilized cell lysate revealed that Keg1 binds to Kre6, which has been known to participate in beta-1,6-glucan synthesis. To analyze the essential function of Keg1 in more detail, we constructed temperature-sensitive mutant alleles by error-prone polymerase chain reaction. The keg1-1 mutant cells showed a common phenotype with Deltakre6 mutant including hypersensitivity to Calcofluor white, reduced sensitivity to the K1 killer toxin, and reduced content of beta-1,6-glucan in the cell wall. These results suggest that Keg1 and Kre6 have a cooperative role in beta-1,6-glucan synthesis in S. cerevisiae.
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Authors | Kosuke Nakamata, Tomokazu Kurita, M Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, Koji Yoda |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 282
Issue 47
Pg. 34315-24
(Nov 23 2007)
ISSN: 0021-9258 [Print] United States |
PMID | 17893149
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzenesulfonates
- Fluorescent Dyes
- K1 killer toxin
- KEG1 protein, S cerevisiae
- KRE6 protein, S cerevisiae
- Killer Factors, Yeast
- Membrane Proteins
- Mycotoxins
- Saccharomyces cerevisiae Proteins
- beta-Glucans
- beta-1,6-glucan
- C.I. Fluorescent Brightening Agent 28
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Topics |
- Benzenesulfonates
(pharmacology)
- Cell Wall
(genetics, metabolism)
- Drug Resistance, Fungal
(drug effects, genetics)
- Endoplasmic Reticulum
(genetics, metabolism)
- Fluorescent Dyes
(pharmacology)
- Killer Factors, Yeast
- Membrane Proteins
(genetics, metabolism)
- Mutation
- Mycotoxins
(pharmacology)
- Protein Binding
(drug effects, physiology)
- Saccharomyces cerevisiae
(genetics, metabolism)
- Saccharomyces cerevisiae Proteins
(genetics, metabolism)
- beta-Glucans
(metabolism)
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