Ly49G2 receptor blockade reduces tumor burden in a leukemia model but not in a solid tumor model.

Abstract

BACKGROUND:

NK cell activity is regulated in part by inhibitory receptors that bind to MHC class I molecules. It is possible to enhance NK cell cytotoxicity against tumor cells by preventing the interaction of these inhibitory receptors with their MHC class I ligands.

RESULTS:

In this study, we determined that Ly49G2 is an inhibitory receptor in AKR mice for self-MHC class I, and AKR Ly49G2 has an identical sequence to BALB/c Ly49G2. Blockade of Ly49G2 receptors in vivo resulted in decreased growth of BW-Sp3 lymphoma cells when the tumor cells were given i.v. but not when the tumor cells were inoculated into the flank forming a solid tumor. However, NK cells were involved in inhibiting the growth of BW-Sp3 tumor cells in the flank.

CONCLUSION:

These data demonstrate that the effectiveness of inhibitory receptor blockade depends upon the tissue location of the tumor cells.

• Authors: Melissa A Barber, Tong Zhang, Bethany A Gagne, Jo A Van Ginderachter, Patrick De Baetselier, Charles L Sentman
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 57 Issue 5 Pg. 655-62 (May 2008) ISSN: 0340-7004 [Print] Germany
• PMID: 17891395 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antigens, Ly
• Lectins, C-Type
• Receptors, NK Cell Lectin-Like

Topics

• Animals
• Antigens, Ly (genetics)
• Base Sequence
• Disease Models, Animal
• Flow Cytometry
• Killer Cells, Natural (immunology)
• Lectins, C-Type (antagonists & inhibitors, genetics)
• Leukemia (immunology, therapy)
• Mice
• Molecular Sequence Data
• Neoplasms, Experimental (immunology, therapy)
• Receptors, NK Cell Lectin-Like
• Reverse Transcriptase Polymerase Chain Reaction