Investigation on clotting and hemolysis characteristics of heparin-immobilized polyether sulfones biomembrane.
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| Abstract |
In this study, a novel heparin-immobilized polyethersulfone (PES) was synthesized. PES was initially sulfonated with chlorosulfonic acid and then 1,6-hexanediamine was grafted to the -SO(3)H groups of sulfonated PES, which subsequently reacted with heparin through a covalent bond by using (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) as catalyst. The hydrophobic/hydrophilic property was characterized by measuring the water contact angle. The data shows decline from 62.29 degrees +/- 1.2 degrees to 47.86 degrees +/- 0.3 degrees for water and 86.79 degrees +/- 0.8 degrees to 68.34 degrees +/- 1.0 degrees for glycerol, which indicates an enhancement of hydrophilicity. Plasma hemolysis assay shows a comparatively low hemolysis ratio of 1.04%, which is below permissible limit of 5%. A higher content of dissociated blood cells and Ca(2+) concentration was found in red blood cell counting and coagulation factor IV test in heparinized PES. Plasma recalcification time of 360 s also offers positive evidence that heparinized PES seems to have a good anticoagulation property. This new heparin-immobilized PES biomaterials may have the potential for biomedical applications.
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| Authors | Changjun Hou, Quan Yuan, Danqun Huo, Shujia Zheng, Dongli Zhan |
| Journal | Journal of biomedical materials research. Part A (J Biomed Mater Res A) Vol. 85 Issue 3 Pg. 847-52 (Jun 01 2008) ISSN: 1552-4965 [Electronic] United States |
| PMID | 17876803 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright 2007 Wiley Periodicals, Inc. |
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