Pre-ischemic administration of landiolol prevents ischemia-reperfusion injury in the rat heart.

Abstract

BACKGROUND:

This study was designed to investigate whether administration of landiolol before or during ischemia protects the myocardium against ischemia-reperfusion (I/R) injury.

METHODS:

Isolated rat hearts were perfused with Krebs-Henseleit buffer using Langendorff apparatus. Cardiac arrest was achieved using St. Thomas' Hospital cardioplegic solution for 3 min. Each heart was subjected to global ischemia at 37 degrees C for 45 min followed by reperfusion for 60 min. In protocol A, landiolol (5-200 microM) was administered before ischemia and in protocol B, landiolol (10-1000 microM) was administered during ischemia. Post-ischemic recovery of cardiac function after 60 min of reperfusion was expressed as a percentage of the pre-ischemic value. Creatinine kinase (CK) leakage during reperfusion for 20 min was also measured.

RESULTS:

Post-ischemic recovery of cardiac function and CK leakage were significantly improved by administration of 100 microM landiolol in protocol A (left ventricular developed pressure, 54.5 +/- 10.5% vs 76.1 +/- 6.6%; dP/dtmax, 47.5 +/- 14.9% vs 71.4 +/- 6.9%; coronary flow, 68.5 +/- 7.1% vs 83.9 +/- 12.9%, and CK, 25.3 +/- 4.2 IU vs 20.3 +/- 7.4 IU). In protocol B, landiolol exerted no cardioprotective effect on cardiac function and CK leakage at each concentration.

CONCLUSIONS:

Landiolol has a cardioprotective effect on I/R injury in the rat heart when administered before ischemia.

• Authors: Yosuke Takahashi, Toshihiko Shibata, Yasuyuki Sasaki, Hidekazu Hirai, Shigekazu Takemura, Yukiko Minamiyama, Masanori Sakaguchi, Shigefumi Suehiro
• Journal: Osaka city medical journal (Osaka City Med J) Vol. 53 Issue 1 Pg. 9-16 (Jun 2007) ISSN: 0030-6096 [Print] Japan
• PMID: 17867629 (Publication Type: Journal Article)

Chemical References

• Adrenergic beta-Antagonists
• Morpholines
• landiolol
• Urea

Topics

• Adrenergic beta-Antagonists (pharmacology)
• Animals
• Dose-Response Relationship, Drug
• Heart (drug effects, physiopathology)
• Ischemic Preconditioning, Myocardial (methods)
• Male
• Morpholines (pharmacology)
• Myocardial Reperfusion Injury (pathology, physiopathology, prevention & control)
• Myocardium (enzymology, pathology)
• Rats
• Rats, Wistar
• Reperfusion Injury (pathology, physiopathology, prevention & control)
• Urea (analogs & derivatives, pharmacology)
• Ventricular Dysfunction, Left (physiopathology)