Abstract | OBJECTIVE: We investigated the effects of three different sites for delivery of bone marrow mesenchymal stem cells (MSCs) in a rat model of myocardial ischemia. DESIGN: Prospective, randomized, controlled study. SETTING: University affiliated research institute. SUBJECTS: Male Sprague-Dawley rats. INTERVENTIONS: MEASUREMENTS AND MAIN RESULTS: Echocardiographically measured myocardial function, including ejection fraction and fractional shortening, was quantitated 2 wks and 4 wks after administering MSCs or phosphate buffer solution. Hemodynamics, including cardiac index, LV dP/dt40, LV negative dP/dt, and LV diastolic pressure were measured 4 wks after administering MSCs or phosphate buffer solution. MSCs were counted in 5-microm sections obtained with cryostat from each harvested heart. Significant improvements in ejection fraction, fractional shortening, cardiac index, LV dP/dt40, LV negative dP/dt, and LV diastolic pressure followed injection of MSCs, regardless of the site of injection. However, the number of MSCs counted in the heart sections was significantly greater after direct myocardial injection. CONCLUSIONS: Independently of the site of injection and regardless of the different concentration of bone marrow mesenchymal stem cells identified in the myocardium, myocardial function was comparably improved in all groups of animals treated with MSCs.
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Authors | Tong Wang, Wanchun Tang, Shijie Sun, Giuseppe Ristagno, Zitong Huang, Max Harry Weil |
Journal | Critical care medicine
(Crit Care Med)
Vol. 35
Issue 11
Pg. 2587-93
(Nov 2007)
ISSN: 0090-3493 [Print] United States |
PMID | 17828039
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Bone Marrow Transplantation
(methods)
- Disease Models, Animal
- Infusions, Intravenous
- Male
- Mesenchymal Stem Cell Transplantation
(methods)
- Myocardial Ischemia
(physiopathology, surgery)
- Rats
- Rats, Sprague-Dawley
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