Abstract | UNLABELLED: METHODS: Three-month-old virgin Wistar strain female rats received VPA (i.p.) at a dose of 300 mg/kg once a day for 12 weeks; control group received an equivalent volume of vehicle. GnRH, GABA and PSA-NCAM expressions were studied by immunohistofluorescence technique from mPOA and ME- ARC region of hypothalamus. Ovarian histology was also studied using Mayer's Haematoxylin- Eosin staining method. RESULTS:
GnRH and PSA-NCAM staining was much higher in mPOA and ME- ARC region from vehicle treated control proestrous rats, whereas VPA treatment significantly enhanced GABA expression, and reduced both GnRH and PSA-NCAM expression. Mayer's Haematoxylin- Eosin staining of mid-ovarian sections revealed significantly higher number of ovarian follicular cysts in VPA treated rats. CONCLUSIONS: Our findings of alterations in GnRH and GABA expression and GnRH neuronal plasticity marker PSA-NCAM as well as changes in ovarian histology suggest that treatment with VPA disrupts hypothalamo-hypophyseal-gonadal axis (HPG) at the level of GnRH pulse generator in hypothalamus.
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Authors | Dinesh Lakhanpal, Gurcharan Kaur |
Journal | Cellular and molecular neurobiology
(Cell Mol Neurobiol)
Vol. 27
Issue 8
Pg. 1069-83
(Dec 2007)
ISSN: 0272-4340 [Print] United States |
PMID | 17823865
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Neural Cell Adhesion Molecule L1
- Sialic Acids
- polysialyl neural cell adhesion molecule
- Gonadotropin-Releasing Hormone
- gamma-Aminobutyric Acid
- Valproic Acid
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Topics |
- Animals
- Estrous Cycle
(drug effects, metabolism)
- Female
- Gonadotropin-Releasing Hormone
(metabolism)
- Neural Cell Adhesion Molecule L1
(metabolism)
- Neurons
(drug effects, metabolism)
- Ovary
(cytology, drug effects)
- Rats
- Rats, Wistar
- Sialic Acids
(metabolism)
- Valproic Acid
(adverse effects, pharmacology)
- gamma-Aminobutyric Acid
(metabolism)
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