| Abstract | Antagonising effects of alpha-ketoglutarate (alpha-KG) could be attributed to complexing of the reactive nucleophile (CN-) to form cyanohydrin in cyanide intoxication. However, an enormous protection obtained could not be delineated on account of possible in situ binding of alpha-KG given intraperitoneally (i.p.) in mice to cyanide administered through the same route. The present study was designed to see the efficacy of alpha-KG alone or in combination with sodium nitrite (SN) and/or sodium thiosulfate (STS) in male mice exposed to cyanide administered through subcutaneous (s.c.) or inhalation route. A technique for generation of hydrogen cyanide (HCN) is also discussed. On the basis of protection index (PI), defined here as the LD50 of cyanide in protected mice/LD50 of cyanide in unprotected mice and survival time, STS + alpha-KG regimen was equipotent to the conventional SN + STS regimen. This is further substantiated by effect of alpha-KG in reducing plasma cyanide levels. The efficacy of alpha-KG remains undeterred irrespective of the route of cyanide intoxication, while the magnitude of protection varies. |
| Authors | R Bhattacharya, R Vijayaraghavan
(Affiliation: Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.)
|
| Journal | Biomedical and environmental sciences : BES
(Biomed Environ Sci)
Vol. 4
Issue 4
Pg. 452-60
(Dec 1991)
ISSN: 0895-3988 UNITED STATES |
| PMID | 1781940
(Publication Type: Journal Article)
|
| Chemical References |
- Ketoglutaric Acids
- Potassium Cyanide
- alpha-ketoglutaric acid
- Methemoglobin
|
| Topics |
- Animals
- Drug Antagonism
- Female
- Ketoglutaric Acids
(pharmacology)
- Lethal Dose 50
- Methemoglobin
(analysis)
- Mice
- Poisoning
(blood, prevention & control)
- Potassium Cyanide
(blood, poisoning)
|
