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Examination of milk samples from experimentally infected heifers by an N-acetyl-beta-D-glucosaminidase test and an antigen-capture ELISA.

Abstract
Milk samples collected from normal and experimentally infected quarters of five heifers throughout their first lactation were examined by bacterial culture, milk cell count (MCC), N-acetyl-beta-D-glucosaminidase (NAGase) test and a monoclonal antibody based antigen-capture ELISA. The results were analysed according to the presence (mastitis positive) or absence (mastitis negative) of bacteria on culture, and the numbers of false negative and false positive results of the other three tests defined in relation to this. Similar numbers of false negative results were observed with the MCC (20), NAGase (18) and ELISA (13). False positive results due to the physiological factors present in early lactation were evident in the MCC, prominent in the NAGase test and absent from the ELISA. The major difference in false positive results associated with experimental infection between the three tests was the more rapid return to negative values of the ELISA following resolution of infection, compared with MCC and NAGase.
AuthorsH J Ball, D Greer, D Finlay, D P Mackie
JournalResearch in veterinary science (Res Vet Sci) Vol. 51 Issue 3 Pg. 299-305 (Nov 1991) ISSN: 0034-5288 [Print] England
PMID1780585 (Publication Type: Journal Article)
Chemical References
  • Antigens, Bacterial
  • Acetylglucosaminidase
Topics
  • Acetylglucosaminidase (analysis)
  • Animals
  • Antigens, Bacterial (analysis)
  • Bacteria (isolation & purification)
  • Cattle
  • Cell Count (veterinary)
  • Clinical Enzyme Tests (veterinary)
  • Enzyme-Linked Immunosorbent Assay (veterinary)
  • Evaluation Studies as Topic
  • Female
  • Mastitis, Bovine (diagnosis, drug therapy)
  • Milk (cytology, enzymology, immunology, microbiology)
  • Predictive Value of Tests
  • Staphylococcal Infections (veterinary)
  • Streptococcal Infections (veterinary)

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