Induction of cytotoxic T lymphocytes specific to malignant glioma using T2 cells pulsed with HLA-A2-restricted interleukin-13 receptor alpha 2 peptide in vitro.

Abstract

Interleukin-13 receptor alpha2 (IL-13Ralpha2) is a glioma-restricted cell-surface epitope not otherwise detected within the central nervous system. The present study is a report of a novel approach of targeting malignant glioma with IL-13Ralpha2-specific cytotoxic T lymphocyte (CTL) induced from the peripheral blood mononuclear cells of healthy donors by multiple stimulations with human leukocyte antigen (HLA)-A2-restricted IL-13Ralpha2(345-353) peptide-pulsed T2 cells. The induced CTL showed specific lysis against T2 cells pulsed with the peptide and HLA-A2+ glioma cells expressing IL-13R2(345-353), while HLA-A2 glioma cell lines that express IL-13Ralpha2(345-353) could not be recognized by CTL. The peptide-specific activity was inhibited by anti-HLA class I monoclonal antibody. These results suggest that the induced CTL specific for IL-13Ralpha2(345-353) peptide could be a potential target of specific immunotherapy for HLA-A2 patients with malignant glioma.

• Authors: Xiaobing Jiang, Xiaoling Lu, Ru'en Liu, Fangcheng Zhang, Hongyang Zhao
• Journal: Acta biochimica et biophysica Sinica (Acta Biochim Biophys Sin (Shanghai)) Vol. 39 Issue 9 Pg. 641-8 (Sep 2007) ISSN: 1672-9145 [Print] China
• PMID: 17805458 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-A2 Antigen
• Interleukin-13 Receptor alpha2 Subunit
• Peptides

Topics

• Cell Line
• Cell Line, Tumor
• Coculture Techniques
• Glioma (immunology, pathology)
• HLA-A2 Antigen (immunology)
• Humans
• Immunophenotyping
• Interleukin-13 Receptor alpha2 Subunit (biosynthesis, genetics, immunology)
• Lymphocyte Activation (immunology)
• Peptides (immunology, metabolism)
• T-Lymphocytes, Cytotoxic (immunology, metabolism)