Abstract |
Fumonisins B1 and B2 and AAL toxin are a series of structurally related mycotoxins. Fumonisins B1 and B2, produced by Fusarium moniliforme Sheldon induce toxic hepatitis and hepatomas in rats and leukoencephalomalacia in horses. The cancer-promotion assay which has been used to guide their purification is slow and consumes large amounts of sample. We have examined a series of cultured mammalian cell lines in order to develop a more rapid and sensitive bioassay system, which may be useful for examining structure-activity relationships and the mechanism(s) of action of these toxins. Of 9 rat hepatoma cell lines tested, all except the two most de-differentiated line were sensitive to the three toxins, with a toxic response visible by 48 h. Approximate IC50 values for the most sensitive hepatoma line, H4TG, were 4, 2 and 10 micrograms/ml for fumonisins B1, B2 and AAL toxin, respectively, in 100 microliters cultures. Among 15 cell lines from other sources, only MDCK dog kidney epithelial cells were sensitive (IC50 = 2.5, 2 and 5 micrograms/ml, respectively). Studies in co-cultures of sensitive and insensitive cell lines and in cultures of a sensitive cell line over a range of cell densities indicated that cytotoxicity of fumonisins B1 and B2 does not involve metabolite activation to a derivative stable enough to diffuse to adjacent cells.
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Authors | W T Shier, H K Abbas, C J Mirocha |
Journal | Mycopathologia
(Mycopathologia)
Vol. 116
Issue 2
Pg. 97-104
(Nov 1991)
ISSN: 0301-486X [Print] Netherlands |
PMID | 1780003
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Carcinogens, Environmental
- Fumonisins
- Mycotoxins
- fumonisin B2
- fumonisin B1
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Topics |
- Alternaria
- Animals
- Carcinogens, Environmental
(toxicity)
- Cell Division
(drug effects)
- Cell Line
- Cell Survival
(drug effects)
- Dose-Response Relationship, Drug
- Fumonisins
- Humans
- Mycotoxins
(toxicity)
- Tumor Cells, Cultured
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