Expression of retinoid X receptor alpha is decreased in 3'-methyl-4-dimethylaminoazobenzene-induced hepatocellular carcinoma in rats.

Abstract

The identification of the specific molecular targets, which underlie liver carcinogenesis is essential for the establishment of an effective strategy for the prevention and/or treatment of hepatocellular carcinomas (HCCs). We previously found that a malfunction of RXRalpha due to its aberrant phosphorylation was associated with the development of HCCs. However, it has remained unclear whether the abnormalities in the expression of RXRalpha or the other retinoid receptors play a role in the early stage of liver carcinogenesis. The present study was designed to determine whether alterations in the expression of RXRalpha and the other retinoid receptors RARalpha and RARbeta are involved in hepatocarcinogenesis using a 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB)-induced rat liver carcinogenesis model. We found that immunohistochemical expression of RXRalpha was decreased in liver cell tumors (HCCs and adenoma) and glutathione S-transferase placental form (GST-P)-positive foci, which is a precancerous lesion of HCC, when compared with the non-cancerous tissues. Western blot and RT-PCR analyses revealed a progressive decrease in the expression levels of RXRalpha, RARalpha, and RARbeta proteins and their mRNAs in 3'-MeDAB-induced HCCs and their surrounding tissues, when compared with the normal liver tissues from the control group. Moreover, the expression level of beta-catenin, the heterodimeric partner for both RXRalpha and RARalpha, was immunohistochemically observed in the cytoplasm and, in some cases, in the nucleus of HCC cells. The nuclear expression of cyclin D1, the downstream target molecule of beta-catenin, was also increased in HCC cells when compared with their adjacent normal appearing tissues. Our findings suggest that loss of retinoid receptors, especially RXRalpha, plays a critical role in the chemically-induced rat liver carcinogenesis and this might be associated with the activation of beta-catenin-related signaling pathway.

• Authors: Nobuhiro Ando, Masahito Shimizu, Masataka Okuno, Rie Matsushima-Nishiwaki, Hisashi Tsurumi, Takuji Tanaka, Hisataka Moriwaki
• Journal: Oncology reports (Oncol Rep) Vol. 18 Issue 4 Pg. 879-84 (Oct 2007) ISSN: 1021-335X [Print] Greece
• PMID: 17786350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Rara protein, rat
• Receptors, Retinoic Acid
• Retinoic Acid Receptor alpha
• Retinoid X Receptor alpha
• beta Catenin
• retinoic acid receptor beta
• Cyclin D1
• Methyldimethylaminoazobenzene
• Glutathione S-Transferase pi
• Gstp1 protein, rat

Topics

• Adenoma (chemically induced, metabolism, pathology)
• Animals
• Blotting, Western
• Carcinoma, Hepatocellular (chemically induced, metabolism, pathology)
• Cell Nucleus
• Cyclin D1 (metabolism)
• Gene Expression Regulation, Neoplastic
• Glutathione S-Transferase pi (metabolism)
• Immunoenzyme Techniques
• Liver Neoplasms, Experimental (metabolism, pathology)
• Male
• Methyldimethylaminoazobenzene (toxicity)
• RNA, Messenger
• Rats
• Rats, Inbred F344
• Receptors, Retinoic Acid (metabolism)
• Retinoic Acid Receptor alpha
• Retinoid X Receptor alpha (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• beta Catenin (metabolism)