[Effect of Na(+)/Ca(2+) exchange blocker, Benzamil, on alveolar macrophage function in patients with chronic obstructive pulmonary disease].

Abstract

OBJECTIVE:

To observe the effect of Benzamil on cytosolic free calcium ions ([Ca(2+)] i) concentration, tumor necrosis factor-alpha (TNF-alpha) and malondialdehyde (MDA) in alveolar macrophage (AM) in patients with chronic obstructive pulmonary disease (COPD).

METHODS:

Alveolar macrophages were collected from bronchoalveolar lavage fluid, and they were cultured and [Ca(2+)] i were determined by calcium fluorescent with indicator Fura-2/AM. The effects of Benzamil on the increase in cytosolic [Ca(2+)] i and on secretion of TNF-alpha, MDA were investigated in 36 patients with COPD and 36 healthy volunteers.

RESULTS:

(1) [Ca(2+)] i concentration (68.26+/-7.24) nmol/L, TNF-alpha (5.74+/-0.42) ng/L and MDA (3.77+/-0.61) microg/L were found in COPD patients, and they were significantly increased, as compared with the control group, in whom the contents were [Ca(2+)] i concentration (60.61+/-6.26) nmol/L, TNF-alpha (2.06+/-0.20) microg/L, MDA (1.91+/-0.19) ng/L (all P<0.01). (2)The following data namely [Ca(2+)] i concentration (168.34+/-17.58) nmol/L, TNF-alpha (9.67+/-1.01) ng/L and MDA (11.21+/-1.01) microg/L were obtained after anoxia in COPD patients (all P<0.01). (3)After incubation with Benzamil, and then subjected the cells to anoxia, the following data were obtained: [Ca(2+)] i concentration (129.21+/-14.33) nmol/L, TNF-alpha content (6.78+/-0.52) ng/L and MDA (8.47+/-0.79) microg/L and they were all lower than only anoxia was applied (all P<0.01).

CONCLUSION:

Benzamil not only downregulates alveolar macrophages activity but also suppresses the generation of the TNF-alpha and MDA.

• Authors: Xu Li, Li-Xiang Wang, Jian-Peng Zhang, Ou-Xiang Gu, Man-Jing Song
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 19 Issue 9 Pg. 522-4 (Sep 2007) ISSN: 1003-0603 [Print] China
• PMID: 17767818 (Publication Type: English Abstract, Journal Article)

Chemical References

• Sodium-Calcium Exchanger
• Tumor Necrosis Factor-alpha
• benzamil
• Malondialdehyde
• Amiloride
• Calcium

Topics

• Aged
• Amiloride (analogs & derivatives, pharmacology)
• Bronchoalveolar Lavage Fluid (cytology)
• Calcium (metabolism)
• Cells, Cultured
• Female
• Humans
• Macrophages, Alveolar (drug effects, metabolism)
• Male
• Malondialdehyde (metabolism)
• Middle Aged
• Pulmonary Disease, Chronic Obstructive (metabolism, pathology)
• Sodium-Calcium Exchanger (antagonists & inhibitors)
• Tumor Necrosis Factor-alpha (metabolism)