Hypoestoxide inhibits tumor growth in the mouse CT26 colon tumor model.

Abstract

AIM:

To evaluate the effect of the natural diterpenoid, hypoestoxide (HE) on the growth of established colon cancer in mice.

METHODS:

The CT26.WT mouse colon carcinoma cell line was grown and expanded in vitro. Following the expansion, BALB/c mice were inoculated s.c. with viable tumor cells. After the tumors had established and developed to about 80-90 mm(3), the mice were started on chemotherapy by oral administration of HE, 5-fluorouracil (5-FU) or combination.

RESULTS:

The antiangiogenic HE has previously been shown to inhibit the growth of melanoma in the B16F(1) tumor model in C57BL/6 mice. Our results demonstrate that mean volume of tumors in mice treated with oral HE as a single agent or in combination with 5-FU, were significantly smaller (> 60%) than those in vehicle control mice (471.2 mm(3) vs 1542.8 mm(3), P < 0.01). The significant reductions in tumor burden resulted in pronounced mean survival times (MST) and increased life spans (ILS) in the treated mice.

CONCLUSION:

These results indicate that HE is an effective chemotherapeutic agent for colorectal cancer in mice and that HE may be used alone or in combination with 5-FU.

• Authors: Emmanuel A Ojo-Amaize, Howard B Cottam, Olusola A Oyemade, Joseph I Okogun, Emeka J Nchekwube
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 13 Issue 34 Pg. 4586-8 (Sep 14 2007) ISSN: 1007-9327 [Print] United States
• PMID: 17729410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Diterpenes
• hypoestoxide
• Fluorouracil

Topics

• Administration, Oral
• Animals
• Antineoplastic Combined Chemotherapy Protocols (pharmacology, therapeutic use)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Colonic Neoplasms (drug therapy, pathology)
• Diterpenes (administration & dosage)
• Dose-Response Relationship, Drug
• Female
• Fluorouracil (administration & dosage)
• Mice
• Mice, Inbred BALB C
• Neoplasms, Experimental (drug therapy, pathology)
• Time Factors