Rapid detection of causative pathogen of peritonitis using in-situ hybridization in a patient with continuous ambulatory peritoneal dialysis.

Abstract

Continuous ambulatory peritoneal dialysis (CAPD) peritonitis is a common problem in patients on peritoneal dialysis, and the bacteriological diagnosis is important for the treatment of this condition. We used Hybrizep (a method for detecting the genes of bacteria ingested in phagocytes), to detect the causative bacterium in a 50-year-old woman receiving peritoneal dialysis who was admitted our hospital with bacterial peritonitis. The test, using peritoneal dialysis fluid, was positive for Streptococcus epidermidis. Vancomycin administration led to a good outcome for this patient. The Hybrizep method (which is available on the Japanese National Health system only for the diagnosis of septicemia) was a useful diagnostic approach for a patient on peritoneal dialysis who had peritonitis.

• Authors: Kyuma Ota, Hiroki Maruyama, Noriaki Iino, Gen Nakamura, Masaaki Shimotori, Yoshinari Tanabe, Hiroki Tsukada, Fumitake Gejyo
• Journal: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy (J Infect Chemother) Vol. 13 Issue 4 Pg. 273-5 (Aug 2007) ISSN: 1341-321X [Print] Netherlands
• PMID: 17721693 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Vancomycin

Topics

• Anti-Bacterial Agents (therapeutic use)
• Female
• Humans
• In Situ Hybridization (methods)
• Middle Aged
• Peritoneal Dialysis, Continuous Ambulatory (adverse effects)
• Peritonitis (diagnosis, microbiology)
• Staphylococcal Infections (diagnosis, drug therapy, genetics)
• Staphylococcus epidermidis (drug effects, genetics, pathogenicity)
• Vancomycin (therapeutic use)