Autoimmunity to deltaNp63alpha in chronic ulcerative stomatitis.

Abstract	Chronic ulcerative stomatitis (CUS) is a recently described mucocutaneous condition in which patients experience chronic, painful, ulcerative lesions of the oral mucosa. CUS is diagnosed by immunofluorescence studies that demonstrate antinuclear antibodies. These autoantibodies are specific for a protein, deltaNp63alpha, which is normally expressed in basal cell nuclei of stratified squamous epithelia. The purpose of this study was to characterize the autoimmune response in CUS. Protein antigens were produced by in vitro transcription/translation of polymerase chain-reaction (PCR)-amplified cDNAs. We used immunoblotting and immunoprecipitation experiments with serum from CUS patients to examine the (1) antibody isotype, (2) immunogenic functional domains of the deltaNp63alpha antigen, and (3) cross-reactivity with homologous p53, p73, and p63 proteins. Results demonstrate CUS patient antibodies to deltaNp63alpha, and 52% of cases have circulating IgA isotype antibodies. The N-terminal and DNA-binding domains are the immunodominant regions, and antibody cross-reactivity with p53, p63, and p73 isoforms is limited.
Authors	L W Solomon, M E Neiders, M G Zwick, K L Kirkwood, V Kumar
Journal	Journal of dental research (J Dent Res) Vol. 86 Issue 9 Pg. 826-31 (Sep 2007) ISSN: 0022-0345 [Print] United States
PMID	17720849 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Autoantibodies DNA-Binding Proteins Immunoglobulin G Protein Isoforms TP63 protein, human Trans-Activators Transcription Factors Tumor Suppressor Protein p53 Tumor Suppressor Proteins
Topics	Autoantibodies (immunology, isolation & purification) Autoimmune Diseases (immunology) Autoimmunity (physiology) Blotting, Western Chronic Disease Cross Reactions DNA-Binding Proteins (chemistry, immunology) Fluorescent Antibody Technique Gingivitis, Necrotizing Ulcerative (blood, immunology) Humans Immunoglobulin G (immunology) Protein Isoforms Protein Structure, Tertiary Trans-Activators (chemistry, immunology) Transcription Factors Tumor Suppressor Protein p53 (immunology) Tumor Suppressor Proteins (chemistry, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!