The effect of the insecticide heptachlor on ras proto-oncogene expression in human myeloblastic leukemia (ML-1) cells.

Abstract

Expression of the ras proto-oncogene mRNA in human myeloblastic leukemia (ML-1) cells was analyzed as a function of cDNA amplification by polymerase chain reaction (PCR). By using a pair of oligonucleotides that flank exon-2 from opposite strands (5' and 3') of H-ras cDNA for PCR amplification, ML-1 cells were found to express a 112 bp segment of the ras transcript. A rapid decline in the expression of this transcript was seen in cells treated with heptachlor, a chlorinated hydrocarbon insecticide. Expression of the same ras segment was not affected by treatment of ML-1 with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Furthermore, addition of serum to quiescent, heptachlor-treated cultures of ML-1 cells inhibited the effect of heptachlor and restored the expression of the ras protooncogene mRNA.

• Authors: L F Chuang, R Y Chuang
• Journal: Toxicology (Toxicology) Vol. 70 Issue 3 Pg. 283-92 ( 1991) ISSN: 0300-483X [Print] Ireland
• PMID: 1771636 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Actins
• Culture Media
• MAS1 protein, human
• Proto-Oncogene Mas
• RNA, Messenger
• Heptachlor
• Tetradecanoylphorbol Acetate

Topics

• Actins (genetics)
• Base Sequence
• Blood
• Culture Media
• Gene Expression (drug effects)
• Genes, ras
• Heptachlor (pharmacology)
• Humans
• Leukemia, Myeloid, Acute (genetics)
• Molecular Sequence Data
• Polymerase Chain Reaction
• Proto-Oncogene Mas
• RNA, Messenger (genetics)
• Tetradecanoylphorbol Acetate (pharmacology)
• Tumor Cells, Cultured