Wound infections, especially those associated with methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa, offer considerable challenges for clinicians. Our laboratory has recently developed novel composite
biomaterials (DRDC) for
wound dressing applications, and demonstrated their in vitro bactericidal efficacy. In the present study, we assessed the proliferation of planktonic and sessile Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus in porcine full-thickness
wounds covered for up to 48 h with either saline- or
mafenide acetate-loaded DRDC puffs and meshes. All
biomaterials were applied 4 h following bacterial inoculation of the
wounds with methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa, to allow colonization of the tissues and initiation of biofilm formation. The
drug-loaded
biomaterials eradicated both the planktonic and biofilm bacteria in the
wounds within 24 h (p <. 05), irrespective of the bacterial strain or architecture of the dressing. While the
wound bioburdens increased in the ensuing 24 h, they remained approximately 2 log(10) colony-forming units (CFU) below (p <. 05) their respective baseline values. Similarly, less than 4 log(10) CFU was recovered in the
drug-loaded DRDC
biomaterials throughout the study. These data show that the DRDC puffs and meshes are effective in delivering certain medications, such as
antimicrobial agents, to the
wound bed, suggesting considerable value of this material for treating
wounds, especially those with irregular shapes, contours, and depths.