An apolipoprotein A-II polymorphism (-265T/C, rs5082) regulates postprandial response to a saturated fat overload in healthy men.

Abstract

Apolipoprotein (Apo) A-II is an apolipoprotein with an unknown role in lipid metabolism. It has been suggested that the presence of the less frequent allele of a single nucleotide polymorphism (Apo A-II -265T/C, rs5082) reduces the transcription rate of Apo A-II and enhances VLDL postprandial clearance in middle-aged men. To further investigate the role of Apo A-II -265T/C on lipid metabolism, we studied 88 normolipidemic young men. The participants were given a fatty meal containing 1 g fat and 7 mg cholesterol/kg weight and capsules containing 60,000 IU vitamin A (retinyl palmitate, 15.15 mg RE) per square meter body surface area. Postprandial lipemia was assessed during the 11 h following the meal. Total cholesterol (Chol) and triacylglycerols (TG) in plasma and TG-rich lipoproteins (TRL) (large TRL and small TRL) were measured, as well as HDL, Apo A-I, Apo B, Apo B-48, and Apo B-100. Postprandial responses were higher in the TT group than in carriers of the minor allele (CC/TC) for total TG in plasma (21.37% of change of area under curve, P = 0.014), large TRL-TG (24.75% change, P = 0.017) and small TRL-Chol (26.63% change, P = 0.003). Our work shows that carriers of the minor allele for Apo A-II -265T/C (CC/TC) have a lower postprandial response compared with TT homozygotes. This finding may partially explain the role of Apo A-II in lipid metabolism and can identify a population with a decreased risk of cardiovascular disease, as corresponds to the lower level of postprandial hypertriglyceridemia.

• Authors: Javier Delgado-Lista, Francisco Perez-Jimenez, Toshiko Tanaka, Pablo Perez-Martinez, Yolanda Jimenez-Gomez, Carmen Marin, Juan Ruano, Laurence Parnell, Jose Maria Ordovas, Jose Lopez-Miranda
• Journal: The Journal of nutrition (J Nutr) Vol. 137 Issue 9 Pg. 2024-8 (Sep 2007) ISSN: 0022-3166 [Print] United States
• PMID: 17709437 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein A-II
• Fats
• Cytosine
• Thymine

Topics

• Adolescent
• Adult
• Apolipoprotein A-II (genetics)
• Cytosine (metabolism)
• Fats (pharmacology)
• Health
• Humans
• Lipid Metabolism
• Male
• Postprandial Period (drug effects)
• Thymine (metabolism)