[Correlation between expression of apoptosis-related gene pnas-2 and leukemia].

Abstract

The study was purposed to explore the correlation between apoptosis-related gene pnas-2 and leukemia. The RT-PCR was performed to detect the expression levels of pnas-2 gene in NB4, K562, U937 cells before and after treatment with AS(4)S(4), and to analysis the expression change of pnas-2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas-2 gene in arsenic sulfide treated NB4 cells was down regulated in time-dependent manner, but the same outcome in K562 and U937 cells after being treated with AS(4)S(4) was not found. The positive expression rate of pnas-2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher than that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in no-remission patients there were no significant differences of expression of pnas-2 before and after treatment. It is concluded that the pnas-2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as a molecular biological remission marker in acute leukemia.

• Authors: Hong-Hui Huang, Jian-Yi Zhu, Ji-Hua Zhong, Hai-Rong Wang, Hua Zhong, Jie-Ying Han, Fang-Yuan Chen
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 15 Issue 4 Pg. 738-42 (Aug 2007) ISSN: 1009-2137 [Print] China
• PMID: 17708794 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Regulatory Proteins
• Arsenicals
• PNAS-2 protein, human
• Sulfides
• arsenic trisulfide

Topics

• Adolescent
• Adult
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Arsenicals (pharmacology)
• Female
• Humans
• K562 Cells
• Leukemia (pathology)
• Leukemia, Myeloid, Acute (drug therapy, genetics, pathology)
• Male
• Middle Aged
• Sulfides (pharmacology)
• Tumor Cells, Cultured
• U937 Cells
• Young Adult