Protective effect of pharmacological preconditioning of total flavones of abelmoschl manihot on cerebral ischemic reperfusion injury in rats.

Abstract	The present study was to investigate the effect of pharmacological preconditioning of total flavones of abelmoschl manihot (TFA) on cerebral ischemic reperfusion injury in rats. Rat cerebral ischemia/reperfusion injury was induced by occluding the right middle cerebral artery (MCA). The infarct size was determined by staining with 2,3,5-triphenyl tetrazalium chloride (TTC). The serum malonaldehyde (MDA), nitric oxide (NO) and lactate dehydrogenase (LDH) levels were measured by using spectrophotometry; Inducible NO synthase (iNOS) mRNA expression was detected by RT-PCR method. The percentage of cerebral infarction volume was 28.1 +/- 0.8 in the model group, while TFA or nimodipine (Nim) pretreatment 36 hours prior to the ischemic insult significantly decreased the infarction volume. Increases of serum LDH activity and MDA level were observed after ischemia/reperfusion, but these changes were inhibited in rats pretreated with either TFA (20, 40, 80, 160 mg/kg) or Nim, indicating a delayed protective effect of TFA preconditioning on cerebral ischemic reperfusion injury. In addition, the serum NO level and the cerebral iNOS mRNA were up-regulated, suggesting a possible mechanism for the protective effect of TFA pretreatment on cerebral ischemic reperfusion injury.
Authors	Ji-Yue Wen, Zhi-Wu Chen
Journal	The American journal of Chinese medicine (Am J Chin Med) Vol. 35 Issue 4 Pg. 653-61 ( 2007) ISSN: 0192-415X [Print] Singapore
PMID	17708631 (Publication Type: Journal Article)
Chemical References	Drugs, Chinese Herbal Flavones RNA, Messenger Nitric Oxide Malondialdehyde L-Lactate Dehydrogenase Nitric Oxide Synthase Type II
Topics	Abelmoschus Animals Brain Ischemia (prevention & control) Cerebral Infarction (metabolism, pathology) Disease Models, Animal Drugs, Chinese Herbal (therapeutic use) Female Flavones (therapeutic use) Ischemic Preconditioning (methods) L-Lactate Dehydrogenase (blood) Lipid Peroxidation (drug effects) Male Malondialdehyde (blood) Nitric Oxide (blood) Nitric Oxide Synthase Type II (metabolism) RNA, Messenger (metabolism) Rats Reperfusion Injury (prevention & control)

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