Characterization of the MUC1.Tg/MIN transgenic mouse as a model for studying antigen-specific immunotherapy of adenomas.

Abstract

A bigenic MUC1.Tg/MIN mouse model was developed by crossing Apc/(MIN/+) (MIN) mice with human MUC1 transgenic mice to evaluate MUC1 antigen-specific immunotherapy of intestinal adenomas. The MUC1.Tg/MIN mice developed adenomas at a rate comparable to that of MIN mice and had similar levels of serum MUC1 antigen. A MUC1-based vaccine consisting of MHC class I-restricted MUC1 peptides, a MHC class II-restricted pan-helper peptide, unmethylated CpG oligodeoxynucleotide and GM-CSF caused flattening of adenomas and significantly reduced the number of large adenomas. Immunization was successful in generating a MUC1-directed immune response evidenced by increased MUC1 peptide-specific anti-tumor cytotoxicity and IFN-gamma secretion by lymphocytes.

• Authors: Emmanuel T Akporiaye, Deborah Bradley-Dunlop, Sandra J Gendler, Pinku Mukherjee, Cathy S Madsen, Tobias Hahn, David G Besselsen, Sharon M Dial, Haiyan Cui, Katrina Trevor
• Journal: Vaccine (Vaccine) Vol. 25 Issue 39-40 Pg. 6965-74 (Sep 28 2007) ISSN: 0264-410X [Print] Netherlands
• PMID: 17707958 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Adjuvants, Immunologic
• CPG-oligonucleotide
• Cancer Vaccines
• MUC1 tandem repeat peptide
• Mucin-1
• Oligodeoxyribonucleotides
• Peptide Fragments
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Adenoma (immunology, pathology, therapy)
• Adjuvants, Immunologic
• Amino Acid Sequence
• Animals
• Cancer Vaccines (administration & dosage, immunology)
• Disease Models, Animal
• Granulocyte-Macrophage Colony-Stimulating Factor (administration & dosage, immunology)
• Humans
• Immunotherapy
• Intestinal Neoplasms (immunology, pathology, therapy)
• Male
• Mice
• Mice, Transgenic
• Molecular Sequence Data
• Mucin-1 (administration & dosage, chemistry, genetics, immunology)
• Oligodeoxyribonucleotides (administration & dosage, immunology)
• Peptide Fragments (administration & dosage, chemistry, genetics, immunology)
• Vaccination