Up-regulation of CXC chemokines and their receptors: implications for proinflammatory microenvironments of ovarian carcinomas and endometriosis.

Abstract

Molecular abnormalities in the epithelial cells of endometriosis and their relevance to carcinogenesis of the ovary have been well studied. On the other hand, the differences of proinflammatory microenvironments between endometriosis and ovarian carcinomas have not been well documented yet. In this study, the expression patterns of CXC chemokines (IL-8, ENA-78, GRO-alpha, I-TAC, Mig, and SDF-1) and their receptors (CXCR2, CXCR3, and CXCR4) were compared among 12 ovarian carcinomas, 8 endometriosis, and 6 normal ovaries using quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry. The CXCR3-mediated signaling in ovarian carcinoma cells in vitro was also investigated. In quantitative reverse transcriptase polymerase chain reaction, ENA-78 was up-regulated both in endometriosis and carcinomas, whereas I-TAC was detected exclusively in carcinomas. CXCR3 was up-regulated both in carcinomas and endometriosis. However, immunohistochemical studies revealed that the localization of CXCR3 in carcinomas was distinctively different from that in endometriosis. In carcinoma-endometriosis coexisting cases, CXCR3-positive lymphocytes in benign lesions decreased in proportion as CXCR3-positive tumor cells replaced the tissues. CXCR3 was also detected in ovarian carcinoma cell lines in vitro. Administration of interferon gamma (IFN-gamma)-inducible chemokines induced extracellular signal-regulated kinase phosphorylation in these carcinoma cells. The results indicated that CXC chemokines might contribute to the progression of ovarian carcinomas and endometriosis in different manners. Aberrant expression of IFN-gamma-inducible chemokines and CXCR3 in carcinoma cells in association with reduced CXCR3-positive immune cells raised the possibility that IFN-gamma-inducible chemokines might not exert effective antitumor immune responses but that they might work in favor of tumor progression.

• Authors: Mitsuko Furuya, Takahito Suyama, Hirokazu Usui, Yoshitoshi Kasuya, Mariko Nishiyama, Naotake Tanaka, Isamu Ishiwata, Yuichiro Nagai, Makio Shozu, Sadao Kimura
• Journal: Human pathology (Hum Pathol) Vol. 38 Issue 11 Pg. 1676-87 (Nov 2007) ISSN: 0046-8177 [Print] United States
• PMID: 17707463 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL1 protein, human
• CXCL11 protein, human
• CXCL12 protein, human
• CXCL5 protein, human
• CXCL9 protein, human
• CXCR3 protein, human
• Chemokine CXCL1
• Chemokine CXCL11
• Chemokine CXCL12
• Chemokine CXCL5
• Chemokine CXCL9
• Chemokines, CXC
• Interleukin-8
• Receptors, CXCR
• Receptors, CXCR3
• Receptors, CXCR4

Topics

• Adult
• Aged
• Cell Line, Tumor
• Chemokine CXCL1 (biosynthesis)
• Chemokine CXCL11 (biosynthesis)
• Chemokine CXCL12 (biosynthesis)
• Chemokine CXCL5 (biosynthesis)
• Chemokine CXCL9 (biosynthesis)
• Chemokines, CXC (biosynthesis)
• Endometriosis (physiopathology)
• Female
• Humans
• Immunohistochemistry
• Interleukin-8 (biosynthesis)
• Middle Aged
• Ovarian Neoplasms (physiopathology)
• Ovary (metabolism)
• Receptors, CXCR (biosynthesis)
• Receptors, CXCR3 (biosynthesis)
• Receptors, CXCR4 (biosynthesis)
• Up-Regulation