[Overexpression of estrogen receptor-related receptor alpha can stimulate estrogen receptor negative endometrial cancer cell proliferation].

Abstract

OBJECTIVE:

To investigate the role of human estrogen receptor-related receptor (ERR) alpha, a submember of orphan receptors, in the tumorigenesis of endometrial cancer.

METHODS:

Plasmid of pSG-ERRalpha was transfected into endometrial cancer cell lines HEC-1A, HEC-1B, and Ishikawa. Real-time quantitative RT-PCR and western blot were used to analyze the mRNA and protein expression of ERRalpha in endometrial cancer cell. Flow cytometry was used to analyze the cellular growth.

RESULTS:

Expressions of the ERRalpha were significantly increased in the endometrial cancer cells transfected with pSG-ERRalpha plasmid; expression of the ERRalpha mRNA in HEC-1A cell was 9644.4 copies/ng, HEC-1B: 9835.3 copies/ng, and Ishikawa: 8008.6 copies/ng (P < 0.01); expression of the ERRalpha protein in HEC-1A cell was 1.128, HEC-1B: 1.104, and Ishikawa: 1.008 (P < 0.05). Flow cytometry showed over-expression of ERRalpha was accompanied by increased HEC-1A and HEC-1B cells in S and G(2)/M phase (P < 0.01), while this could not be observed in the estrogen receptor (ER) positive endometrial cancer cell line Ishikawa. Furthermore, cellular growth analysis showed that over-expression of ERRalpha induced cell growth increase of the ER negative endometrial cancer cells HEC-1A and HEC-1B (P < 0.05).

CONCLUSION:

Over-expression of ERRalpha could stimulate ER negative endometrial cancer cell proliferation independent of estrogen-ER pathway.

• Authors: Peng-Ming Sun, Li-Hui Wei, Min Gao, Jian-Liu Wang, Li-Jun Zhao, Da-Peng Wang, Jun-Xiao Zhang
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 42 Issue 6 Pg. 408-11 (Jun 2007) ISSN: 0529-567X [Print] China
• PMID: 17697604 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ERRalpha estrogen-related receptor
• RNA, Messenger
• Receptors, Estrogen

Topics

• Blotting, Western
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation
• Endometrial Neoplasms (genetics, metabolism, pathology)
• Female
• Flow Cytometry
• Gene Expression Regulation, Neoplastic
• Humans
• Plasmids
• RNA, Messenger (biosynthesis, genetics)
• Receptors, Estrogen (biosynthesis, genetics, physiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Transfection