Abstract |
Pseudomonas aeruginosa is an opportunistic pathogen that causes a number of infections in humans, but is best known for its association with cystic fibrosis. It is able to use a wide range of sulfur compounds as sources of sulfur for growth. Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. These genes were localized in five distinct islands on the genome and encoded proteins with a significantly reduced content of cysteine and methionine. Growth of P. aeruginosa E601 with mucin as the sulfur source led not only to a sulfate starvation response but also to induction of genes involved with type III secretion systems.
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Authors | Tewes Tralau, Stéphane Vuilleumier, Christelle Thibault, Barry J Campbell, C Anthony Hart, Michael A Kertesz |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 189
Issue 19
Pg. 6743-50
(Oct 2007)
ISSN: 0021-9193 [Print] United States |
PMID | 17675390
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Gene Expression Profiling
- Gene Expression Regulation, Bacterial
(drug effects)
- Genome, Bacterial
- Mucins
(pharmacology)
- Oligonucleotide Array Sequence Analysis
- Pseudomonas aeruginosa
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Sulfates
(pharmacology)
- Transcription, Genetic
(drug effects)
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