[Comparison of the proteomes of isoniazid-resistant Mycobacterium tuberculosis strains and isoniazid-susceptible strains].

Abstract

OBJECTIVE:

To determine whether there is a major difference in protein levels between isoniazid-resistant and susceptible M. tuberculosis strains.

METHODS:

The proteins extracted from 9 isoniazid-resistant M. tuberculosis strains and 7 isoniazid-susceptible M. tuberculosis strains were analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Protein spots remarkly increased in isoniazid-resistant M. tuberculosis strains were digested in gel by enzyme and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The data obtained from peptide mass fingerprinting were used in protein database search. These genes of all strains were sequenced.

RESULT:

In all protein spot differences, 5 protein spots were upregulated in isoniazid-resistant strains and identified as Rv1446c, Rv3028c, Rv0491, Rv2971, and Rv2145 by (MALDI-TOF-MS).

CONCLUSION:

These results suggest that the differentially expressed proteins from isoniazid-resistant strains might be used as potential immuno-diagnostic antigens and candidate novel drug targets against drug resistant tuberculosis.

• Authors: Xin Jiang, Feng Gao, Wen-hong Zhang, Zhong-yi Hu, Hong-hai Wang
• Journal: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases (Zhonghua Jie He He Hu Xi Za Zhi) Vol. 30 Issue 6 Pg. 427-31 (Jun 2007) ISSN: 1001-0939 [Print] China
• PMID: 17673015 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antitubercular Agents
• Bacterial Proteins
• Isoniazid

Topics

• Antitubercular Agents (pharmacology)
• Bacterial Proteins (genetics, metabolism)
• Databases, Protein
• Drug Resistance, Bacterial
• Electrophoresis, Gel, Two-Dimensional
• Humans
• Isoniazid (pharmacology)
• Microbial Sensitivity Tests
• Mycobacterium tuberculosis (drug effects, genetics, metabolism)
• Proteomics (methods)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Tuberculosis, Multidrug-Resistant (drug therapy, microbiology)