Early drought-induced changes to the needle proteome of Norway spruce.

Abstract

To elucidate early drought responses in needles of Norway spruce (Picea abies (Karst.) L.), we subjected 1-year-old seedlings to gradual desiccation for 6 weeks. Four weeks of drought treatment caused a small but significant decrease in photosystem II quantum yield of light-adapted needles (phi(a)) compared with that of well-watered controls. Six weeks of drought treatment reduced phi(a) and the photosystem II quantum yield of dark-adapted needles (phi) by 50 and 8%, respectively, and reduced shoot water potential by 0.7 MPa, but had no measurable effect on needle relative water content. After two weeks of drought treatment, and before there was a discernible effect of drought on phi or a statistically significant effect on shoot water potential, needles were analyzed for changes in protein composition. Five out of several hundred detected proteins in needles of drought-treated plants showed consistent changes compared with control leaves. The proteins were identified by LC-MS/MS as components of the oxygen-evolving complex (oxygen evolving enhancer protein 2), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, and one protein of unknown function, whose mRNA was found in a previous screen of wound- and methyl-jasmonate-induced bark proteins.

• Authors: Constanze Blödner, Andrzej Majcherczyk, Ursula Kües, Andrea Polle
• Journal: Tree physiology (Tree Physiol) Vol. 27 Issue 10 Pg. 1423-31 (Oct 2007) ISSN: 0829-318X [Print] Canada
• PMID: 17669733 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Proteins
• Water

Topics

• Disasters
• Gene Expression Profiling
• Gene Expression Regulation, Plant
• Picea (metabolism)
• Plant Leaves (metabolism)
• Plant Proteins (genetics, metabolism)
• Water (metabolism)