Abstract | BACKGROUND: STUDY DESIGN AND METHODS:
Glycoprotein ( GP) IIb/IIIa was purified by affinity chromatography from outdated PLT concentrates derived from HPA-1aa or HPA-1bb donors. Purified GPs were biotinylated, immobilized onto streptavidin beads, and used for the analysis of HPA-1a alloantibodies by a microtyping system. HPA-1a serum samples derived from mothers with NAIT (n = 36) and from posttransfusion purpura patients (n = 2) as well as HPA-1b (n = 4), HPA-5b (n = 2), HPA-3a (n = 4), and HLA Class I (n = 2) alloantiserum samples from multitransfused patients were investigated in GASA and MAIPA assays. RESULTS: GASA was able to detect all HPA-1a and -1b alloantibodies recognized by MAIPA. Cross-reactivity with other PLT-reactive alloantibodies was not observed. Interestingly, 3 of 36 serum samples, which showed only moderate reactivity in MAIPA, reacted strongly in GASA. CONCLUSION: GASA has proved to be a rapid method for the detection of HPA-1a alloantibodies and maybe useful for PLT antibody screening, especially in initial assessment of suspected NAIT cases.
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Authors | Tamam Bakchoul, Oliver Meyer, Ashraf Agaylan, Stephane Bombard, Gregor Bein, Ulrich J H Sachs, Abdulgabar Salama, Sentot Santoso |
Journal | Transfusion
(Transfusion)
Vol. 47
Issue 8
Pg. 1363-8
(Aug 2007)
ISSN: 0041-1132 [Print] United States |
PMID | 17655579
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Human Platelet
- Isoantibodies
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Topics |
- Antibodies, Monoclonal
(immunology)
- Antigens, Human Platelet
(immunology)
- Humans
- Infant, Newborn
- Isoantibodies
(blood)
- Microspheres
- Purpura, Thrombocytopenic, Idiopathic
(diagnosis)
- Sensitivity and Specificity
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