Abstract |
Asthma is an inflammatory lung disease, in which CD1d-restricted natural killer T (NKT) cells play an important pathogenic role. Also, recent reports indicated that a cytokine, thymic stromal lymphopoietin (TSLP), is essential for the development of antigen-induced asthma. Here we examined the relationship between NKT cells and TSLP in a mouse model of asthma. NKT cells express TSLP receptor as well as IL-7 receptor alpha-chain. TSLP acts on NKT cells to preferentially increase their IL-13 production but not IFN-gamma and IL-4. In an allergen-induced asthma model, the development of airway hyperreactivity, a cardinal feature of asthma, was increased in TSLP transgenic mice, whereas this effect was not observed in TSLP transgenic mice lacking NKT cells. Interestingly, in the NKT cell-lacking TSLP transgenic mice, pulmonary eosinophilia and increase in IgE did not improve. Pulmonary lymphocytes from the NKT cell-lacking TSLP transgenic mice produced much less IL-13 upon CD3 stimulation than those from NKT cell-competent TSLP transgenic mice. These resultssuggest that, in allergen-induced asthma, TSLP acts on NKT cells to enhance airway hyperreactivity by upregulating their IL-13 production, whereas eosinophilia and IgE production are not influenced.
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Authors | Yuko Nagata, Hajime Kamijuku, Masaru Taniguchi, Steven Ziegler, Ken-ichiro Seino |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 144
Issue 4
Pg. 305-14
( 2007)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 17652941
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2007 S. Karger AG, Basel. |
Chemical References |
- Cytokines
- Immunoglobulins
- RNA, Messenger
- Receptors, Cytokine
- Recombinant Proteins
- Tslpr protein, mouse
- Immunoglobulin E
- Ovalbumin
- Thymic Stromal Lymphopoietin
- TSLP protein, mouse
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Topics |
- Animals
- Asthma
(chemically induced, immunology, physiopathology)
- Bronchial Hyperreactivity
(chemically induced, immunology, physiopathology)
- Bronchoalveolar Lavage Fluid
(cytology)
- Cell Proliferation
(drug effects)
- Cytokines
(genetics, immunology, pharmacology)
- Immunoglobulin E
(blood, immunology)
- Immunoglobulins
- Killer Cells, Natural
(cytology, drug effects, immunology)
- Lung
(immunology, pathology, physiopathology)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Ovalbumin
(immunology)
- RNA, Messenger
(metabolism)
- Receptors, Cytokine
(genetics, metabolism)
- Recombinant Proteins
(immunology, pharmacology)
- Th2 Cells
(immunology)
- Thymic Stromal Lymphopoietin
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