[Study of trisomy 22 and inversion 16 in acute myeloid leukemia].

Abstract

OBJECTIVE:

To explore the value of trisomy 22 ( +22) in the diagnosis of inv(16) acute myeloid leukemia (AML).

METHODS:

Interphase fluorescence in situ hybridization (FISH) was performed in 18 AML patients with +22. The probe was two-color break apart probe for CBFbeta with SpectrumRed on the centromeric side and SpectrumGreen on the telomeric side. The FISH results were compared with that of R-banding conventional cytogenetics (CC). Multiplex FISH (M-FISH) was used to analyze the relationship of +22 and inv(16).

RESULTS:

CC revealed inv(16) in none of the 18 AML, with +22, but FISH revealed inv (16) in 11 of them and del( 16) (q22) in one. As CC results, 9 of the 11 cases were sole +22, one complicated with trisomy 8, and one del(16) (q22). Four patients with +22 and inv(16) were analyzed by M-FISH and revealed +22 only.

CONCLUSION:

+22 can be regarded as an important marker for the diagnosis of inv(16) AML.

• Authors: Hui-fen Zhou, Jian-yong Li, Jin-lan Pan, Hai-rong Qiu, Li-juan Chen, Jie-ying Hu, Yun-feng Shen, Wei Xu, Yong-quan Xue
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 28 Issue 1 Pg. 11-4 (Jan 2007) ISSN: 0253-2727 [Print] China
• PMID: 17649718 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adolescent
• Adult
• Aged
• Chromosome Inversion
• Chromosomes, Human, Pair 16 (genetics)
• Chromosomes, Human, Pair 22 (genetics)
• Female
• Humans
• In Situ Hybridization, Fluorescence
• Leukemia, Myeloid, Acute (genetics)
• Male
• Middle Aged
• Trisomy