Abstract |
The effects of recombinant gamma interferon (IFN gamma) on proliferation, estrogen-receptor (ER) content, mRNA level and protein secretion of a breast cancer estrogen-induced protein pS2/BCEI were investigated in two human breast cancer cell lines, ZR75-1 and T47D. Both cell lines have estrogen and progesterone receptors and previously showed HLA class I and class II responses to IFN gamma (Int J Cancer 1990; 45: 1169). An antiproliferative effect of IFN gamma (1000-5000 U/ml) was observed in serum containing medium on ZR75-1 but not on T47D cells. Noninhibitory concentration of IFN gamma (100 U/ml) had sensitising antiproliferative effect with antiestrogens on ZR75-1 cells whereas IFN gamma did not modify the growth inhibition observed in T47D cells with antiestrogens. In serum-free, estradiol-free, phenol-red-free chemically defined medium ( Cancer Res 1984; 44: 4553), IFN gamma abolished in ZR75-1 but not in T47D the 30% growth stimulation induced by estradiol. In ZR75-1 cells, IFN gamma induced a transitory 30-50% increase of ER content, as measured by ER-enzymoimmunoassay, at day 2 of culture, and reduced mRNA level and secretion of pS2/BCEI. In T47D cells, a 30-50% decrease of ER content was observed but only when cells were long term cultured (30 weeks) with IFN gamma. In this cell line, no transcription of pS2/BCEI was observed. Antiproliferative action of IFN gamma on ZR75-1 cells is associated with an inhibition of estradiol effects and a reduction of pS2/BCEI mRNA level and protein secretion.
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Authors | E Solary, J F Prud'homme, C Gauville, H Magdelenat, F Calvo |
Journal | Journal of biological regulators and homeostatic agents
(J Biol Regul Homeost Agents)
1991 Jul-Sep
Vol. 5
Issue 3
Pg. 98-106
ISSN: 0393-974X [Print] Italy |
PMID | 1763658
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Neoplasm Proteins
- Proteins
- RNA, Messenger
- RNA, Neoplasm
- Receptors, Estradiol
- Recombinant Proteins
- TFF1 protein, human
- Trefoil Factor-1
- Tumor Suppressor Proteins
- Serine
- Interferon-gamma
- Uridine
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Topics |
- Breast Neoplasms
(metabolism, pathology, therapy)
- Cell Division
(drug effects)
- Cell Line
- Female
- Humans
- Interferon-gamma
(pharmacology)
- Neoplasm Proteins
(genetics, metabolism)
- Proteins
- RNA, Messenger
(genetics, metabolism)
- RNA, Neoplasm
(genetics, metabolism)
- Receptors, Estradiol
(drug effects, metabolism)
- Recombinant Proteins
- Serine
(metabolism)
- Trefoil Factor-1
- Tumor Cells, Cultured
(drug effects, metabolism, pathology)
- Tumor Suppressor Proteins
- Uridine
(metabolism)
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