A neurodegenerative
tauopathy endemic to the Caribbean island of Guadeloupe has been associated with the consumption of anonaceous plants that contain
acetogenins, potent lipophilic inhibitors of complex I of the mitochondrial respiratory chain. To test the hypothesis that
annonacin, a prototypical acetogenin, contributes to the etiology of the disease, we investigated whether
annonacin affects the cellular distribution of the
protein tau. In primary cultures of rat striatal neurons treated for 48 h with
annonacin, there was a concentration-dependent decrease in
ATP levels, a redistribution of tau from the axons to the cell body, and cell death.
Annonacin induced the retrograde transport of mitochondria, some of which had tau attached to their outer membrane.
Taxol, a drug that displaces tau from microtubules, prevented the somatic redistribution of both mitochondria and tau but not cell death.
Antioxidants, which scavenged the
reactive oxygen species produced by complex I inhibition, did not affect either the redistribution of tau or cell death. Both were prevented, however, by forced expression of the NDI1
nicotinamide adenine dinucleotide (
NADH)-quinone-oxidoreductase of Saccharomyces cerevisiae, which can restore
NADH oxidation in complex I-deficient mammalian cells and stimulation of energy production via anaerobic glycolysis. Consistently, other
ATP-depleting
neurotoxins (1-methyl-4-phenylpyridinium, 3-nitropropionic, and carbonyl cyanide m-chlorophenylhydrazone) reproduced the somatic redistribution of tau, whereas toxins that did not decrease
ATP levels did not cause the redistribution of tau. Therefore, the
annonacin-induced
ATP depletion causes the retrograde transport of mitochondria to the cell
soma and induces changes in the intracellular distribution of tau in a way that shares characteristics with some
neurodegenerative diseases.