3-methyl-1,2-cyclopentanedione down-regulates age-related NF-kappaB signaling cascade.
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| Abstract |
Activation of the redox-sensitive transcription factor, nuclear factor-kappa B (NF-kappaB), plays a central role in inflammation and aging processes by inducing pro-inflammatory genes. The present study was designed to unravel the molecular mechanisms underlying the anti-inflammation effects of 3-methyl-1,2-cyclopentanedione (3-MCP) in coffee extracts. In particular, we investigated the effects of 3-MCP on the modulation of NF-kappaB signaling pathways and its target genes in the kidneys of aged animal rats: young (6 months old), old (21 months old), and old 3-MCP-fed (4 and 8 mg/kg/day for 10 days). The results strongly show that 3-MCP exerted potent anti-inflammatory effects, significantly reducing (i) the phosphorylation of inhibitor kappaB (IkappaB) and other NF-kappaB upstream events; (ii) the translocation of NF-kappaB into the nucleus; (iii) the expression of iNOS and COX-2; and (iv) pro-inflammatory genes such as VCAM-1, MCP-1, and IL-6. Furthermore, 3-MCP suppressed reactive oxygen species levels. Taken together, our results clearly demonstrate that 3-MCP modulated the age-related NF-kappaB signaling cascade and its pro-inflammatory genes. Therefore, 3-MCP is proposed to be an effective anti-inflammatory agent that can be a novel approach for the therapy of inflammation.
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| Authors | Jae Heun Chung, Seo Young Choi, Ji Young Kim, Dae Hyun Kim, Jae Won Lee, Jae Sue Choi, Hae Young Chung |
| Journal | Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 55 Issue 16 Pg. 6787-92 (Aug 08 2007) ISSN: 0021-8561 [Print] United States |
| PMID | 17629301 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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