A dot-immunobinding assay for the laboratory diagnosis of tuberculous meningitis and its comparison with enzyme-linked immunosorbent assay.

In an attempt to establish an alternative to standard bacteriological methods in the laboratory diagnosis of tuberculous meningitis (TBM), a simple dot-immunobinding assay (Dot-Iba) was standardized to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of patients with TBM. Sensitivity and specificity of Dot-Iba was compared with conventional enzyme-linked immunosorbent assay (ELISA) and standard bacteriological techniques. The Dot-Iba showed excellent correlation with indirect ELISA for the detection of antimycobacterial antibody in CSF and showed 60% sensitivity and 100% specificity in culture-negative patients with TBM. However Dot-Iba was less sensitive for the detection of antigen 5 in CSFs and showed false negative results (60%) in culture-positive patients with TBM.
AuthorsV V Radhakrishnan, A Mathai
JournalThe Journal of applied bacteriology (J Appl Bacteriol) Vol. 71 Issue 5 Pg. 428-33 (Nov 1991) ISSN: 0021-8847 [Print] ENGLAND
PMID1761436 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Antibodies, Bacterial (cerebrospinal fluid)
  • Antigens, Bacterial (cerebrospinal fluid)
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunoblotting
  • Mycobacterium tuberculosis (immunology)
  • Predictive Value of Tests
  • Tuberculosis, Meningeal (diagnosis)

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