Abstract |
In an attempt to establish an alternative to standard bacteriological methods in the laboratory diagnosis of tuberculous meningitis (TBM), a simple dot-immunobinding assay (Dot-Iba) was standardized to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of patients with TBM. Sensitivity and specificity of Dot-Iba was compared with conventional enzyme-linked immunosorbent assay (ELISA) and standard bacteriological techniques. The Dot-Iba showed excellent correlation with indirect ELISA for the detection of antimycobacterial antibody in CSF and showed 60% sensitivity and 100% specificity in culture-negative patients with TBM. However Dot-Iba was less sensitive for the detection of antigen 5 in CSFs and showed false negative results (60%) in culture-positive patients with TBM.
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Authors | V V Radhakrishnan, A Mathai |
Journal | The Journal of applied bacteriology
(J Appl Bacteriol)
Vol. 71
Issue 5
Pg. 428-33
(Nov 1991)
ISSN: 0021-8847 [Print] England |
PMID | 1761436
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
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Topics |
- Antibodies, Bacterial
(cerebrospinal fluid)
- Antigens, Bacterial
(cerebrospinal fluid)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunoblotting
- Mycobacterium tuberculosis
(immunology)
- Predictive Value of Tests
- Tuberculosis, Meningeal
(diagnosis)
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