Screening for trans-acting factors and other factors involved in the activating or silencing of the gamma-globin gene during human ontogeny.

Researchers hope to increase gamma-globin expression by controlling potential trans-acting factors that specifically activate the gamma-globin gene in fetuses or silence this gene in adults to potentially treat sickle cell disease and beta-thalassemias. To characterize genes encoding such factors, we analyzed the differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal adult bone marrow, umbilical cord blood, and bone marrow from a patient with heterocellular hereditary persistence of fetal hemoglobin. Using differential-display - reverse-transcription PCR analysis, we identified a number of genes with differential expression in the above-mentioned cells. The differential expression of some genes was also confirmed by quantitative real-time PCR. Our data provide important clues for identifying and validating trans-activators that activate the gamma-globin gene in fetuses, and trans-acting factors involved in silencing the gamma-globin gene in adults.
AuthorsYan-Ni Ma, Xin Zhang, Jun-Wu Zhang, Xin-Hua Zhang, Rong-Xin Wang
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire (Biochem Cell Biol) Vol. 85 Issue 3 Pg. 347-57 (Jun 2007) ISSN: 0829-8211 [Print] Canada
PMID17612629 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, CD34
  • DNA Primers
  • RNA, Messenger
  • Trans-Activators
  • Globins
  • Adult
  • Antigens, CD34 (metabolism)
  • Base Sequence
  • Bone Marrow Cells (cytology, immunology, metabolism)
  • Cells, Cultured
  • DNA Primers (genetics)
  • Erythropoiesis (genetics)
  • Expressed Sequence Tags
  • Fetal Blood (cytology, immunology, metabolism)
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Globins (genetics)
  • Humans
  • In Vitro Techniques
  • Infant, Newborn
  • RNA, Messenger (genetics, metabolism)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators (metabolism)

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