Abstract | OBJECTIVE: METHODS: HPMCs were cultured from human omentum by an enzyme digestion method, growing in medium containing 30 mmol/L D-glucose. TGF-beta(1) and Fn expression were measured in HPMCs in the presence and absence of 15 micromol/L troglitazone. The mRNA expressions of PPAR-gamma,TGF-beta(1) and Fn were determined by semi-quantification reverse transcriptive PCR (RT-PCR). The protein of TGF-beta(1) was determined by enzyme-linked immunosorbent assay (ELISA) and proteins of PPAR-gamma and Fn were determined by Western blot. RESULTS: The mRNA and protein expression of TGF-beta(1) and Fn were significantly increased in HPMCs stimulated with 30 mmol/L D-glucose compared with the control group with F12 media (P<0.01). Obvious decrease of TGF-beta(1) was found in troglitazone(15 micromol/L) treated group compared with group stimulated with 30 mmol/L D-glucose (P<0.05). Exposure of HPMCs to troglitazone reduced the Fn secretion (P<0.05). CONCLUSION:
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Authors | Hong Liu, You-ming Peng, Fu-you Liu, Ying-hong Liu, Ling-yan Li, Jun Li, Xing Chen |
Journal | Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
(Zhong Nan Da Xue Xue Bao Yi Xue Ban)
Vol. 32
Issue 3
Pg. 473-9
(Jun 2007)
ISSN: 1672-7347 [Print] China |
PMID | 17611328
(Publication Type: Journal Article)
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Chemical References |
- Chromans
- Fibronectins
- PPAR gamma
- RNA, Messenger
- Thiazolidinediones
- Transforming Growth Factor beta1
- Troglitazone
- Glucose
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Topics |
- Blotting, Western
- Cells, Cultured
- Chromans
(pharmacology)
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Epithelial Cells
(cytology, drug effects, metabolism)
- Fibronectins
(biosynthesis, genetics)
- Glucose
(pharmacology)
- Humans
- PPAR gamma
(biosynthesis, genetics)
- Peritoneum
(cytology)
- RNA, Messenger
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Thiazolidinediones
(pharmacology)
- Transforming Growth Factor beta1
(biosynthesis, genetics)
- Troglitazone
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