Macelignan protects HepG2 cells against tert-butylhydroperoxide-induced oxidative damage.

Abstract

In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.

• Authors: Jong Hee Sohn, Kyu Lee Han, Jeong Han Choo, Jae-Kwan Hwang
• Journal: BioFactors (Oxford, England) (Biofactors) Vol. 29 Issue 1 Pg. 1-10 ( 2007) ISSN: 0951-6433 [Print] Netherlands
• PMID: 17611289 (Publication Type: Journal Article)

Chemical References

• Lignans
• Reactive Oxygen Species
• Malondialdehyde
• macelignan
• tert-Butylhydroperoxide
• L-Lactate Dehydrogenase

Topics

• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Comet Assay
• DNA Damage (drug effects)
• Dose-Response Relationship, Drug
• Humans
• L-Lactate Dehydrogenase (metabolism)
• Lignans (chemistry, pharmacology)
• Lipid Peroxidation (drug effects)
• Malondialdehyde (metabolism)
• Molecular Structure
• Myristica
• Oxidation-Reduction (drug effects)
• Oxidative Stress (drug effects)
• Reactive Oxygen Species (metabolism)
• Time Factors
• tert-Butylhydroperoxide (pharmacology)