Abstract |
In this study, polyamine oxidase from maize (MPAO), which is involved in the terminal catabolism of spermidine and spermine to produce an aminoaldehyde, 1,3-diaminopropane and H(2)O(2), has been conditionally expressed at high levels in the nucleus of MCF-7 human breast cancer cells, with the aim to interfere with polyamine homeostasis and cell proliferation. Recombinant MPAO expression induced accumulation of a high amount of 1,3-diaminopropane, an increase of putrescine levels and no alteration in the cellular content of spermine and spermidine. Furthermore, recombinant MPAO expression did not interfere with cell growth of MCF-7 cells under normal conditions but it did confer higher growth sensitivity to etoposide, a DNA topoisomerase II inhibitor widely used as antineoplastic drug. These data suggest polyamine oxidases as a potential tool to improve the efficiency of antiproliferative agents despite the difficulty to interfere with cellular homeostasis of spermine and spermidine.
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Authors | L Marcocci, M Casadei, C Faso, A Antoccia, P Stano, S Leone, B Mondovì, R Federico, P Tavladoraki |
Journal | Amino acids
(Amino Acids)
Vol. 34
Issue 3
Pg. 403-12
(Apr 2008)
ISSN: 1438-2199 [Electronic] Austria |
PMID | 17610126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Polyamines
- Recombinant Proteins
- Etoposide
- Oxidoreductases Acting on CH-NH Group Donors
- polyamine oxidase
- Doxycycline
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology, therapeutic use)
- Breast Neoplasms
(drug therapy, genetics, pathology)
- Cell Line, Tumor
- Cell Nucleus
(genetics)
- Cell Proliferation
(drug effects)
- Doxycycline
(pharmacology)
- Etoposide
(pharmacology, therapeutic use)
- Gene Expression Regulation, Enzymologic
(drug effects)
- Humans
- Oxidoreductases Acting on CH-NH Group Donors
(genetics, metabolism)
- Polyamines
(metabolism)
- Recombinant Proteins
(genetics, metabolism)
- Zea mays
(enzymology, genetics)
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