Abstract |
We have identified the primary endogenous fluorescent substance, which has characteristic excitation/emission maxima at 380/440 nm and 400/460 nm, found in the sera of patients with chronic renal failure (Clin Chem 32: 1276, 1988). Preliminary studies, using thin layer chromatography (with cellulose) in conjunction with pteridine standards, indicated that the compound is an unconjugated pteridine. Characterization by gas chromatography-mass spectrometry (electron impact), direct probe-mass spectrometry (electron impact/chemical ionization), and Fourier Transform Infrared analysis showed this compound to be xanthopterin (2-amino 4,6 pteridinedione), an unconjugated pteridine known to be present in man in trace quantities. An authentic sample of this compound had a retention time with high-performance liquid chromatography (HPLC) identical to that of the purified fluorophore. The physiological role of xanthopterin in the pathogenesis of uremia has yet to be elucidated.
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Authors | R H Williams, M Shaykh, S Ahmed, T Musiala, N Bazilinski, G Dunea, A Dubin |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 24
Issue 5
Pg. 407-15
(Oct 1991)
ISSN: 0009-9120 [Print] United States |
PMID | 1760879
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Chromatography, Thin Layer
- Humans
- Kidney Failure, Chronic
(blood)
- Spectrum Analysis
- Xanthopterin
(chemistry, isolation & purification)
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