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Purification and biochemical characterization of xanthopterin from patients with chronic renal failure. II. Biochemical elucidation and structural analysis.

Abstract
We have identified the primary endogenous fluorescent substance, which has characteristic excitation/emission maxima at 380/440 nm and 400/460 nm, found in the sera of patients with chronic renal failure (Clin Chem 32: 1276, 1988). Preliminary studies, using thin layer chromatography (with cellulose) in conjunction with pteridine standards, indicated that the compound is an unconjugated pteridine. Characterization by gas chromatography-mass spectrometry (electron impact), direct probe-mass spectrometry (electron impact/chemical ionization), and Fourier Transform Infrared analysis showed this compound to be xanthopterin (2-amino 4,6 pteridinedione), an unconjugated pteridine known to be present in man in trace quantities. An authentic sample of this compound had a retention time with high-performance liquid chromatography (HPLC) identical to that of the purified fluorophore. The physiological role of xanthopterin in the pathogenesis of uremia has yet to be elucidated.
AuthorsR H Williams, M Shaykh, S Ahmed, T Musiala, N Bazilinski, G Dunea, A Dubin
JournalClinical biochemistry (Clin Biochem) Vol. 24 Issue 5 Pg. 407-15 (Oct 1991) ISSN: 0009-9120 [Print] United States
PMID1760879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Xanthopterin
Topics
  • Chromatography, Thin Layer
  • Humans
  • Kidney Failure, Chronic (blood)
  • Spectrum Analysis
  • Xanthopterin (chemistry, isolation & purification)

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