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Purification and biochemical characterization of xanthopterin from patients with chronic renal failure. I. Isolation, purification and preliminary characterization.

Abstract
We describe the purification and initial characterization of a hitherto unrecognized fluorescence (excitation/emission maxima at 380/440 nm and 400/460 nm) reported from this laboratory in patients with chronic renal failure (Clin Chem 31: 1988, 1985). Purification was achieved using Sephadex G-10 gel chromatography combined with reverse phase and ion exchange high-performance liquid chromatography (HPLC). Purity of the "blue-green" fluorescent compound was determined to be greater than 99% by HPLC, and two-dimensional thin layer chromatography using an acidic and basic solvent system. The excitation/emission maxima were shown to be 390 nm/456 nm, and ultraviolet scans, at pH 1.0, 7.0, and 13.0, gave absorbance optima at 261 nm/356 nm, 278 nm/390 nm, and 255 nm/394 nm, respectively. The isoelectric point of 4.05 in conjunction with the fluorescent and ultraviolet spectra suggests that the fluorophore belongs to the class of compounds known as pteridines.
AuthorsR H Williams, M Shaykh, S Ahmed, T Musiala, N Bazilinski, G Dunea, A Dubin
JournalClinical biochemistry (Clin Biochem) Vol. 24 Issue 5 Pg. 399-406 (Oct 1991) ISSN: 0009-9120 [Print] United States
PMID1760878 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Xanthopterin
Topics
  • Chromatography, High Pressure Liquid
  • Humans
  • Kidney Failure, Chronic (blood)
  • Spectrum Analysis
  • Xanthopterin (chemistry, isolation & purification)

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