Abstract |
We describe the purification and initial characterization of a hitherto unrecognized fluorescence (excitation/emission maxima at 380/440 nm and 400/460 nm) reported from this laboratory in patients with chronic renal failure (Clin Chem 31: 1988, 1985). Purification was achieved using Sephadex G-10 gel chromatography combined with reverse phase and ion exchange high-performance liquid chromatography (HPLC). Purity of the "blue-green" fluorescent compound was determined to be greater than 99% by HPLC, and two-dimensional thin layer chromatography using an acidic and basic solvent system. The excitation/emission maxima were shown to be 390 nm/456 nm, and ultraviolet scans, at pH 1.0, 7.0, and 13.0, gave absorbance optima at 261 nm/356 nm, 278 nm/390 nm, and 255 nm/394 nm, respectively. The isoelectric point of 4.05 in conjunction with the fluorescent and ultraviolet spectra suggests that the fluorophore belongs to the class of compounds known as pteridines.
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Authors | R H Williams, M Shaykh, S Ahmed, T Musiala, N Bazilinski, G Dunea, A Dubin |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 24
Issue 5
Pg. 399-406
(Oct 1991)
ISSN: 0009-9120 [Print] United States |
PMID | 1760878
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Chromatography, High Pressure Liquid
- Humans
- Kidney Failure, Chronic
(blood)
- Spectrum Analysis
- Xanthopterin
(chemistry, isolation & purification)
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