B cell lymphoma-associated chromosomal translocation involves candidate oncogene lyt-10, homologous to NF-kappa B p50.

Abstract

A B cell lymphoma-associated chromosomal translocation, t(10;14)(q24;q32), juxtaposes the immunoglobulin C alpha 1 locus to a novel gene, lyt-10. The normal lyt-10 cDNA codes for a 98 kd protein which displays amino-terminal homology with the rel (DNA-binding) domain of the NF-kappa B-rel family of transcription factors and carboxy-terminal homology with the NF-kappa B p50 precursor protein, including the putative proteolytic cleavage domain (poly-G) and the ankyrin-like repeat domains. The lyt-10 protein can bind to kappa B sequences in vitro, although with different specificity from NF-kappa B p50, and in vitro DNA-binding is activated by removal of the ankyrin domain. Chromosomal translocation generates an lyt-10-C alpha 1 fusion gene coding for a protein that retains the rel effector domain, lacks the ankyrin regulatory domain, and binds kappa B sequences in vitro, suggesting its constitutive activation in vivo. Analogous rearrangements of the lyt-10 gene have been found in an additional three cases of lymphoid neoplasia. The lyt-10 gene defines a new subfamily (rel/poly-G/ankyrin) of NF-kappa B-rel transcription factors with potential for oncogenic activation in human cancer.

• Authors: A Neri, C C Chang, L Lombardi, M Salina, P Corradini, A T Maiolo, R S Chaganti, R Dalla-Favera
• Journal: Cell (Cell) Vol. 67 Issue 6 Pg. 1075-87 (Dec 20 1991) ISSN: 0092-8674 [Print] United States
• PMID: 1760839 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA-Binding Proteins
• Immunoglobulin alpha-Chains
• NF-kappa B
• Oligodeoxyribonucleotides
• RNA, Messenger
• Transcription Factors
• DNA

Topics

• Amino Acid Sequence
• Base Sequence
• Chromosomes, Human, Pair 10
• Chromosomes, Human, Pair 14
• Cloning, Molecular
• DNA (genetics)
• DNA-Binding Proteins (genetics)
• Gene Expression
• Gene Rearrangement, B-Lymphocyte, Heavy Chain
• Genes, Immunoglobulin
• Humans
• Immunoglobulin alpha-Chains (genetics)
• Lymphoma, B-Cell (genetics)
• Molecular Sequence Data
• Multigene Family
• NF-kappa B (metabolism)
• Oligodeoxyribonucleotides (chemistry)
• Oncogenes
• Polymerase Chain Reaction
• RNA, Messenger (genetics)
• Regulatory Sequences, Nucleic Acid
• Restriction Mapping
• Transcription Factors (genetics)
• Translocation, Genetic