Effects of cyclosporine A on chick osteoclasts in vitro.

Abstract

We have studied the effects of cyclosporine A (CsA) on basal and bovine parathyroid hormone (1-34) (bPTH)-stimulated bone resorption by osteoclasts in 24-hour cultures of chick long bone cells. At a high concentration (10 micrograms/ml), CsA had a cytotoxic effect on both osteoclasts and mononuclear cells in the culture. At 1 microgram/ml, CsA inhibited basal and bPTH-stimulated bone resorption but was not cytotoxic over 24 hours. We also studied the binding of bPTH to the osteoblastic cell line, Saos-2, and chick long bone cells in suspension culture. CsA inhibited bPTH binding in Saos-2 in a dose-dependent manner; inhibition of binding was also observed in chick bone cells. The effects of CsA on osteoclast viability and resorptive function may be due to a direct effect on the osteoclasts and/or to an interaction with the nonosteoclastic cell population in the culture.

• Authors: M H Chowdhury, V Shen, D W Dempster
• Journal: Calcified tissue international (Calcif Tissue Int) Vol. 49 Issue 4 Pg. 275-9 (Oct 1991) ISSN: 0171-967X [Print] United States
• PMID: 1760772 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Parathyroid Hormone
• Peptide Fragments
• parathyroid hormone (1-34), bovine
• Cyclosporine

Topics

• Analysis of Variance
• Animals
• Bone Resorption
• Cell Count
• Cell Line
• Cell Survival (drug effects)
• Cells, Cultured
• Chickens
• Cyclosporine (pharmacology)
• Osteoclasts (drug effects, metabolism, physiology)
• Parathyroid Hormone (metabolism, pharmacology)
• Peptide Fragments (metabolism, pharmacology)