Abstract | OBJECTIVE: METHODS: Wistar rats were randomized into normal group (n = 8), burn(n =40) , burn and SB203580(n = 16), burn and isotonic saline( n = 16) groups, with 8 rats at each time-points. There were 5 time-points in burn group, and 2 time-points in other groups. The rats in the latter 3 groups were inflicted with 40% TBSA full-thickness burns, and those in burn and SB203580, burn and isotonic saline groups were administered with SB203580 ( p38 MAPK inhibitor) or isotonic saline, respectively. The levels of cPLA2 mRNA and membrane phospholipids in myocardium were detected with RT-PCR. In the same experiment, the effect of SB203580 on cPLA2 expression in rat myocardial cells was determined after hypoxia and burn serum treatment in vitro. RESULTS: The level of myocardial cPLA2 mRNA in burn group at each time-point was obviously higher than those in normal group (0. 280 +/- 0. 020) , and it reached the peak value at 3 PBH. In contrast, the level of cardiac membrane phospholipids was lowered immediately after burns, and it reached the lowest level at 6 PBH [(0. 052 +/- 0. 017) mg phosphorus/mg protein]. Herein, a significant negative correlation was showed between the levels of cPLA2 mRNA and cardiac membrane phospholipids ( r = - 0. 53, P < 0. 05). Administration of SB203580, however, inhibited the increased activity of p38 MAP kinase, suppressed the upregulation of cPLA2(72% and 51% of those in burn and saline group, P <0. 01) , and markedly increased the levels of membrane phospholipids in myocardium at 6 and 12 PBH. In addition, treatment of cardiac myocytes with SB203580 also abolished the upregulation of cPLA2 mRNA elicited by hypoxia and burn serum challenge. CONCLUSION:
p38 MAP kinase play an important role in the burn-induced degradation of cardiac membrane phospholipids in rat through the upregulation of myocardial expression of cPLA2 mRNA in the myocardial cells.
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Authors | Jia-ping Zhang, Xi Ying, Yue-sheng Huang, Yong-ming Dang, Dong-xia Zhang, Xiao-dong Li |
Journal | Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
(Zhonghua Shao Shang Za Zhi)
Vol. 23
Issue 1
Pg. 45-8
(Feb 2007)
ISSN: 1009-2587 [Print] China |
PMID | 17605255
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Phospholipids
- RNA, Messenger
- p38 Mitogen-Activated Protein Kinases
- Phospholipases A2
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Topics |
- Animals
- Burns
(metabolism)
- Disease Models, Animal
- Myocytes, Cardiac
(metabolism)
- Phospholipases A2
(metabolism)
- Phospholipids
(metabolism)
- RNA, Messenger
(metabolism)
- Random Allocation
- Rats
- Rats, Wistar
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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